@article{fdi:010019950,
  title = {{N}ear immunity to rice tungro spherical virus achieved in rice by a replicase-mediated resistance strategy},
  author = {{H}uet, {H}. and {M}ahendra, {S}. and {W}ang, {J}. and {S}ivamani, {E}. and {O}ng, {C}.{A}. and {C}hen, {L}. and {K}ochko, {A}lexandre de and {B}eachy, {R}.{N}. and {F}auquet, {C}laude},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice tungro disease is caused by rice tungro bacilliform virus ({RTBV}), which is responsible for the symptoms, and rice tungro spherical virus ({RTSV}), which assists transmission of both viruses by leafhoppers. {T}ransgenic #japonica$ rice plants (#{O}ryza sativa$) were produced containing the {RTSV} replicase ({R}ep) gene in the sense or antisense orientation. {O}ver 70% of the plants contained one to five copies of the {R}ep gene, with integration occurring at a single locus in most cases. {P}lants producing antisense sequences exhibited significant but moderate resistance to {RTSV} (60%) ; accumulation of antisense {RNA} was substantial, indicating that the protection was not of the homology-dependent type. {P}lants expressing the full-length {R}ep gene, as well as a truncated {R}ep gene, in the (+)-sense orientation were 100% resistant to {RTSV} even when challenged with a high level of inoculum. {A}ccumulation of viral {RNA} was low, leading us to conclude that {RTSV} {R}ep-medicated resistance is not protein-mediated but is of the cosuppression type. {R}esistance was effective against geographically distinct {RTSV} isolates. {I}n addition, {RTSV}-resistant transgenic rice plants were unable to assist transmission of {RTBV}. {S}uch transgenic plants could be used in an epidemiological approach to combat the spread of the tungro disease. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{RIZ} ; {MALADIE} {DES} {PLANTES} ; {PHYTOVIRUS} ; {LUTTE} {GENETIQUE} ; {RESISTANCE} {DE} {L}'{HOTE} ; {ENZYME} ; {TRANSFERT} {DE} {GENE} ; {REP} {GENE} ; {REPLICASE}},
  booktitle = {},
  journal = {{P}hytopathology},
  volume = {89},
  numero = {11},
  pages = {1022--1027},
  ISSN = {0031-949{X}},
  year = {1999},
  DOI = {10.1094/{PHYTO}.1999.89.11.1022},
  URL = {https://www.documentation.ird.fr/hor/fdi:010019950},
}