@article{fdi:010019942, title = {{G}enetic analysis of {L}eishmania parasites in {E}cuador : are {L}eishmania ({V}iannia) panamensis and {L}eishmania ({V}.) guyanensis distinct taxa ?}, author = {{B}anuls, {A}nne-{L}aure and {J}onqui{\`e}res, {R}. and {G}uerrini, {F}. and {L}e {P}ont, {F}ran{\c{c}}ois and {B}arrera, {C}. and {E}spinel, {I}. and {G}uderian, {R}. and {E}cheverria, {R}. and {T}ibayrenc, {M}ichel}, editor = {}, language = {{ENG}}, abstract = {{I}n the course of an epidemiologic survey in {E}cuador, the following collection of {L}eishmania stocks was isolated : 28 from patients with clinical signs of leishmaniasis, 2 from sloths, 1 from a dog, and 4 from sand flies. {F}or genetic characterization of these stocks, multilocus enzyme electrophoresis ({MLEE}) and random amplified polymorphic {DNA} ({RAPD}) were used. {T}wenty six of the 35 stocks were identified as either {L}eishmania ({V}.) panamensis or {L}. ({V}.) guyanensis, 2 stocks were identified as {L}. ({V}.) braziliensis, the 2 stocks from sloths showed specific genotypes, and 5 stocks were characterized as hybrids between {L}. ({V}.) braziliensis and {L}. ({V}.) guyanensis. {T}hese data show that genetic diversity of {L}eishmania in {E}cuador is high and that {L}. ({V}.) panamensis guyanensis is the dominant group in this country. {T}he genetic analysis questioned the distinctness between the two species {L}. ({V}.) panamensis and {L}. ({V}.) guyanensis, since {MLEE} and {RAPD} data did not indicate that {L}. ({V}.) panamensis and {L}. ({V}.) guyanensis correspond to distinct monophyletic lines. {P}opulation genetic analysis performed on the {L}. ({V}.) panamensis/guyanensis group favors the hypothesis of a basically clonal population structure.}, keywords = {{LEISHMANIOSE} ; {PARASITE} ; {ANALYSE} {CHIMIQUE} ; {EPIDEMIOLOGIE} ; {CULTURE} {DE} {MICROORGANISME} ; {ISOENZYME} ; {VARIABILITE} {GENETIQUE} ; {DIAGNOSTIC} ; {ETUDE} {EXPERIMENTALE} ; {ANALYSE} {GENETIQUE} ; {TECHNIQUE} {RAPD} ; {ELECTROPHORESE} ; {EQUATEUR}}, booktitle = {}, journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene}, volume = {61}, numero = {5}, pages = {838--845}, ISSN = {0002-9637}, year = {1999}, URL = {https://www.documentation.ird.fr/hor/fdi:010019942}, }