%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES %A Laplaze, L. %A Gherbi, H. %A Frutz, T. %A Pawlowski, K. %A Franche, C. %A Macheix, J.J. %A Auguy, Florence %A Bogusz, Didier %A Duhoux, Emile %T Flavan-containing cells delimit Frankia-infected compartments in Casuarina glauca nodules %D 1999 %L fdi:010019294 %G ENG %J Plant Physiology %@ 0032-0889 %K SYMBIOSE ; PLANTE ; BACTERIE ; COMPOSE PHENOLIQUE ; FLAVONOIDE ; NODULE RACINAIRE ; ANALYSE CHIMIQUE ; ANALYSE GENETIQUE ; TECHNIQUE PCR ; CLHP %K GENE CHS ; INTERACTION PLANTE MICROORGANISME ; HYBRIDATION IN SITU ; HISTOCHIMIE %N 1 %P 113-122 %R 10.1104/pp.121.1.113 %U https://www.documentation.ird.fr/hor/fdi:010019294 %> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_51-52/010019294.pdf %V 121 %W Horizon (IRD) %X We investigated the involvement of polyphenols in the #Casuarina glauca$-#Frankia$ symbiosis. Histological analysis revealed a cell-specific accumulation of phenolics in #C. glauca$ nodule lobes, creating a compartmentation in the cortex. Histochemical and biochemical analyses indicated that these phenolic compounds belong to the flavan class of flavonoids. We show that the same compounds were synthesized in nodules and uninfected roots. However, the amount of each flavan was dramatically increased in nodules compared with uninfected roots. The use of in situ hybridization established that chalcone synthase transcripts accumulate in flavan-containing cells at the apex of the nodule lobe. Our findings are discussed view of the possible role of flavans in plant-microbe interactions. (Résumé d'auteur) %$ 084SYMBIO