@article{fdi:010018989,
  title = {{C}haracterization of a highly replicative intergroup {M}/{O} human immunodeficiency virus type 1 recombinant isolated from a {C}ameroonian patient},
  author = {{P}eeters, {M}artine and {L}i{\'e}geois, {F}lorian and {T}orimiro, {N}. and {B}ourgeois, {A}. and {M}poudi {N}gol{\'e}, {E}. and {V}ergne, {L}aurence and {S}aman, {E}. and {D}elaporte, {E}ric and {S}aragosti, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} {C}ameroonian patient with antibodies reacting simultaneously to human immunodeficiency virus type 1 ({HIV}-1) group {O}- and group {M}-specific {V}3-loop peptides was identified. {I}n order to confirm that this patient was coinfected with both viruses, {PCR}s with {O}- and {M}-specific discriminating primers corresponding to different regions of the genome were carried out with both primary lymphocyte {DNA} and the corresponding viral strains isolated from three consecutive patent samples. {T}he {PCR} data suggested that this patient is coinfected with a group {M} virus and a recombinant {M}/{O} virus. {I}ndeed, only type {M} gag sequences could be amplified, while for the env region, both type {M} and {O} sequences were amplified, from plasma or from {DNA} extracted from primary lymphocytes. {S}equence analysis of a complete recombinant genome isolated from the second sample (97{CA}-{MP}645 virus isolate) revealed two intergroup breakpoints, one in the vpr gene and the second in the long terminal repeat region around the {TATA} box. {C}omparison of the type {M} sequences shared by the group {M} and the recombinant {M}/{O} viruses showed that these sequences were closely related, with only 3% genetic distance, suggesting that the {M} virus was one of the parental viruses. {I}n this report we describe for the first time a recombination event in vivo between viruses belonging to two different groups, leading to a replicative virus. {R}ecombination between strains with such distant lineages (65% overall homology) may contribute substantially to the emergence of new {HIV}-1 variants. {W}e documented that this virus replicates well and became predominant in vitro. {A}t this time, group {O} viruses represent a minority of the strains responsible for the {HIV}-1 pandemic... ({D}'apr{\`e}s r{\'e}sum{\'e} d'auteur)},
  keywords = {{SIDA} ; {VIRUS} ; {DIVERSITE} {GENETIQUE} ; {ANALYSE} {GENETIQUE} ; {TECHNIQUE} {PCR} ; {PHYLOGENIE} ; {TRANSFORMATION} {GENETIQUE} ; {RECOMBINAISON} {GENETIQUE} ; {CAMEROUN}},
  booktitle = {},
  journal = {{J}ournal of {V}irology},
  volume = {79},
  numero = {9},
  pages = {7368--7375},
  ISSN = {0022-538{X}},
  year = {1999},
  URL = {https://www.documentation.ird.fr/hor/fdi:010018989},
}