@article{fdi:010018688,
  title = {{C}ryopreservation of apple shoot tips by encapsulation-dehydration : effect of preculture, dehydration and freezing procedure on shoot regeneration},
  author = {{Z}hao, {Y}. and {W}u, {Y}. and {E}ngelmann, {F}lorent and {Z}hou, {M}. and {Z}hang, {D}. and {C}hen, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}hoot tips sampled on in vitro cultured apple plantlets of 6 accessions of #{M}. domestica$ and one accession of #{M}. robusta$ were successfully cryopreserved using the encapsulation-dehydration technique. {S}hoot tips were excised from plantlets which had been submitted to 3 weeks of cold-acclimation at 5°{C}, 70 d after their last subculture. {A}fter preculture at 5°{C} in media with progressively increased sucrose concentration (0.1 {M}, 0.3 {M} and 0.7 {M}), shoot tips were encapsulated and pregrown in medium with 1.0 {M} sucrose for 1 d, dehydrated for 4 h under the air current of the laminar flow cabinet, thus reaching a moisture content of around 30% (fresh eight basis) and directly immersed in liquid nitrogen. {T}he regeneration rate of cryopreserved apices varied between 70 and 90%, depending on the accession. {U}sing apcies sampled on plantlets which had been maintained on standard medium without subculture for 6 months, sucrose preculture became unnecessary to achieve regrowth after cryopreservation and the dehydration period was shortened. {T}hese experiments showed that the physiological state of the plant material directly affects the results and procedures for cryopreservation of apple shoot tips. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{CULTURE} {IN} {VITRO} ; {CRYOCONSERVATION} ; {DESHYDRATATION} ; {CONGELATION} ; {PLANTULE} ; {REGENERATION} ; {ETUDE} {EXPERIMENTALE} ; {METHODOLOGIE} ; {MATERIEL} {VEGETAL} ; {POMME} ; {APEX} ; {ENCAPSULATION}},
  booktitle = {},
  journal = {{C}ryo-{L}etters},
  volume = {20},
  numero = {2},
  pages = {103--108},
  ISSN = {0143-2044},
  year = {1999},
  URL = {https://www.documentation.ird.fr/hor/fdi:010018688},
}