Horizon 1 1 17 ACLN : Articles dans des revues avec comité de lecture non répertoriées par l'AERES Journal of Virology 1609-1616 PATHOLOGIE VEGETALE PHYTOVIRUS RESISTANCE PLANTE HOTE LUTTE GENETIQUE TRANSFERT DE GENE GENIE GENETIQUE TECHNIQUE G5P PHAGE M13 PROTEINE D'ENVELOPPE EXPRESSION GENIQUE 1999 fdi:010018010 ENG Journal of Virology 0022-538X 2 https://www.documentation.ird.fr/hor/fdi:010018010 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010018010.pdf 73 Horizon (IRD) Geminiviruses are plant viruses with circular single-stranded DNA (ssDNA) genomes encapsidated in double icosahedral particles. Tomato leaf curl geminivirus (ToLCV) requires coat protein (CP) for the accumulation of ssDNA in protopolasts and in plants but not for systemic infection and symptom development in plants. In the absence of CP, infected protoplasts accumulate reduced levels of ssDNA and increased amounts of double-stranded DNA (dsDNA), compared to accumulation in the presence of wild-type virus. To determine whether the gene 5 protein (g5p), an ssDNA binding protein from #Escherichia coli$ phage M13, could restore the accumulation of ssDNA, ToLCV that lacked the CP gene was modified to express g5p or g5p fused to the N-terminal 66 amino acides of CP (CP66:6G:g5). The modified viruses led to the accumulation of wild-type levels of ssDNA. The high levels of dsDNA accumulation during infections with the modified viruses suggested a direct role for CP in viral DNA replication. ToLCV that produced the CP66:6G:g5 protein did not spread efficiently in #Nicotiana benthamiana$ plants, and inoculated plants developed only very mild symptoms. In infected protoplasts, the CP66:6G:g5 protein was immunolocalized to nuclei. We propose that the fusion protein interferes with the function of the BV1 movement and thereby prevents spread of the infection. (Résumé d'auteur) 076MALPLA06