@article{fdi:010018010,
  title = {{A} phage single-stranded {DNA} (ss{DNA}) binding protein complements ss{DNA} accumulation of a geminivirus and interferes with viral movement},
  author = {{P}adidam, {M}. and {B}eachy, {R}.{N}. and {F}auquet, {C}laude},
  editor = {},
  language = {{ENG}},
  abstract = {{G}eminiviruses are plant viruses with circular single-stranded {DNA} (ss{DNA}) genomes encapsidated in double icosahedral particles. {T}omato leaf curl geminivirus ({T}o{LCV}) requires coat protein ({CP}) for the accumulation of ss{DNA} in protopolasts and in plants but not for systemic infection and symptom development in plants. {I}n the absence of {CP}, infected protoplasts accumulate reduced levels of ss{DNA} and increased amounts of double-stranded {DNA} (ds{DNA}), compared to accumulation in the presence of wild-type virus. {T}o determine whether the gene 5 protein (g5p), an ss{DNA} binding protein from #{E}scherichia coli$ phage {M}13, could restore the accumulation of ss{DNA}, {T}o{LCV} that lacked the {CP} gene was modified to express g5p or g5p fused to the {N}-terminal 66 amino acides of {CP} ({CP}66:6{G}:g5). {T}he modified viruses led to the accumulation of wild-type levels of ss{DNA}. {T}he high levels of ds{DNA} accumulation during infections with the modified viruses suggested a direct role for {CP} in viral {DNA} replication. {T}o{LCV} that produced the {CP}66:6{G}:g5 protein did not spread efficiently in #{N}icotiana benthamiana$ plants, and inoculated plants developed only very mild symptoms. {I}n infected protoplasts, the {CP}66:6{G}:g5 protein was immunolocalized to nuclei. {W}e propose that the fusion protein interferes with the function of the {BV}1 movement and thereby prevents spread of the infection. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{PATHOLOGIE} {VEGETALE} ; {PHYTOVIRUS} ; {RESISTANCE} ; {PLANTE} {HOTE} ; {LUTTE} {GENETIQUE} ; {TRANSFERT} {DE} {GENE} ; {GENIE} {GENETIQUE} ; {TECHNIQUE} ; {G}5{P} ; {PHAGE} {M}13 ; {PROTEINE} {D}'{ENVELOPPE} ; {EXPRESSION} {GENIQUE}},
  booktitle = {},
  journal = {{J}ournal of {V}irology},
  volume = {73},
  numero = {2},
  pages = {1609--1616},
  ISSN = {0022-538{X}},
  year = {1999},
  URL = {https://www.documentation.ird.fr/hor/fdi:010018010},
}