@article{fdi:010017856,
  title = {{G}enetic characterization of the nef gene from human immunodeficiency virus type 1 group {M} strains representing genetic subtypes {A}, {B}, {C}, {E}, {F}, {G}, and {H}},
  author = {{J}ubier-{M}aurin, {V}{\'e}ronique and {S}aragosti, {S}. and {P}erret, {J}.{L}. and {M}poudi {N}gol{\'e}, {E}. and {E}su-{W}illiams, {E}. and {M}ulanga, {C}laire and {L}i{\'e}geois, {F}lorian and {E}kwalanga, {M}. and {D}elaporte, {E}ric and {P}eeters, {M}artine},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ost efforts to characterize sequence variation of {HIV} isolates has been directed toward the structural envelope gene. {F}ew studies have evaluated the sequence variability of auxiliary genes such as nef. {I}n this study 41 new {HIV}-1 strains, representing the majority of the described envelope subtypes of {HIV}-1 ({A} to {H}), were genetically characterized in the nef region. {P}hylogenetic analysis showed that 34 strains could be classified in the same subtype in nef and env, and 7 (19%) of the 41 new virus were recombinants. {F}rom two of the seven strains, recombination occurred upstream of the nef gene, whereas for five of the seven strains recombination occurred withn the nef gene with a crossover close to the 5' end of the {LTR} (long terminal repeat). {T}he low intersubtype distance between subtype {B} and {D} in the nef gene confirms previous observations in the pol env, and gag genes, which suggest a commn ancestor for these subtypes. {T}he majority of all the previously described functional domains in the nef gene were relatively conserved among the different subtypes, with only minor differences being observed. {T}he myristoylation signal among the different subtypes, with only minor differences being observed. {T}he myristoylation signal was less conserved for subtype {C}, with one or more amino acid changes being observed at positions 3, 4, and 5. {T}he highly conserved acidic region (positions 62 to 65), critical for the enhancement of viral synthesis with an increased virus growth rate, was less conserved among the subtype {G} strains from our study. {A}t least three epitopic regions of the nef gene have been defined and each can be recognized by {CTL}s under a variety of {HLA} restrictions ; all were also relatively well conserved between the different genetic subtypes. {D}espite the relatively important genetic variation in nef sequences obtained among the different genetic subtypes, functional domains and {CTL} epitopes were relatively well conserved... ({D}'apr{\`e}s r{\'e}sum{\'e} d'auteur)},
  keywords = {{SIDA} ; {VIRUS} ; {ANALYSE} {GENETIQUE} ; {DISTANCE} {GENETIQUE} ; {VARIABILITE} {GENETIQUE} ; {PHYLOGENIE} ; {GENE} {NEF} ; {RECOMBINAISON}},
  booktitle = {},
  journal = {{AIDS} {R}esearch and {H}uman {R}etroviruses},
  volume = {15},
  numero = {1},
  pages = {23--32},
  ISSN = {0889-2229},
  year = {1999},
  DOI = {10.1089/088922299311673},
  URL = {https://www.documentation.ird.fr/hor/fdi:010017856},
}