%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Mukabayire, O.
%A Boccolini, D.
%A Lochouarn, Laurence
%A Fontenille, Didier
%A Besansky, N.J.
%T Mitochondrial and ribosomal internal transcribed spacer (ITS2) diversity of the African malaria vector Anopheles funestus
%D 1999
%L fdi:010017854
%G ENG
%J Molecular Ecology
%@ 0962-1083
%K MOUSTIQUE ; VECTEUR ; PALUDISME ; VARIABILITE GENETIQUE ; POLYMORPHISME GENETIQUE ; MARQUEUR GENETIQUE ; STRUCTURE DE POPULATION
%K ADN RIBOSOMAL ; ADN MITOCHONDRIALI ; INVERSION CHROMOSOMIQUE
%K SENEGAL ; BURKINA FASO ; KENYA
%N 2
%P 289-297
%R 10.1046/j.1365-294X.1999.00567.x
%U https://www.documentation.ird.fr/hor/fdi:010017854
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010017854.pdf
%V 8
%W Horizon (IRD)
%X The pattern of sequence variation in the mitochondrial DNA cytochrome b gene (cyt-b) and ribosomal DNA internal transcribed spacer 2 (ITS2) was examined in #Anopheles funestus$ from Senegal and Burkina Faso in West Africa and Kenya in East Africa. From both West African countries, samples included individuals hypothesized to represent reproductively isolated taxa based upon different karyotypes and behaviours. Analysis of the cyt-b data revealed high haplotypic diversity (86%) and an average pairwise difference per site of 0.42%. Sequence variation was not partitioned by geographical origin or karyotype class. The most common haplotype was sampled across Africa (= 6000 km). Analysis of the ITS2 data revealed one of the longest spacers yet found in anophelines (= 704 bp). In common with other anoheline ITS2 sequences, this one had microsatellites and frequent runs of individual nucleotides. Also in common with data from other anopheline ITS2 studies, the #An. funestus$ sequences were almost monomorphic, with only two rare polymorphisms detected. The results from both markers are congruent and do not support the hypothesis of reproductively isolated chromosomal taxa within #An. funestus$. Whether the lack of support by mitochondrial DNA (mtDNA) and ribosomal DNA (rDNA) sequences is a result of the recent origin of the presumptive taxa, or of the absence of barriers to gene flow, remains to be elucidated, using more rapidly evolving markers such as microsatellites. (Résumé d'auteur)
%$ 052ANOPAL04