@article{fdi:010017854, title = {{M}itochondrial and ribosomal internal transcribed spacer ({ITS}2) diversity of the {A}frican malaria vector {A}nopheles funestus}, author = {{M}ukabayire, {O}. and {B}occolini, {D}. and {L}ochouarn, {L}aurence and {F}ontenille, {D}idier and {B}esansky, {N}.{J}.}, editor = {}, language = {{ENG}}, abstract = {{T}he pattern of sequence variation in the mitochondrial {DNA} cytochrome b gene (cyt-b) and ribosomal {DNA} internal transcribed spacer 2 ({ITS}2) was examined in #{A}nopheles funestus$ from {S}enegal and {B}urkina {F}aso in {W}est {A}frica and {K}enya in {E}ast {A}frica. {F}rom both {W}est {A}frican countries, samples included individuals hypothesized to represent reproductively isolated taxa based upon different karyotypes and behaviours. {A}nalysis of the cyt-b data revealed high haplotypic diversity (86%) and an average pairwise difference per site of 0.42%. {S}equence variation was not partitioned by geographical origin or karyotype class. {T}he most common haplotype was sampled across {A}frica (= 6000 km). {A}nalysis of the {ITS}2 data revealed one of the longest spacers yet found in anophelines (= 704 bp). {I}n common with other anoheline {ITS}2 sequences, this one had microsatellites and frequent runs of individual nucleotides. {A}lso in common with data from other anopheline {ITS}2 studies, the #{A}n. funestus$ sequences were almost monomorphic, with only two rare polymorphisms detected. {T}he results from both markers are congruent and do not support the hypothesis of reproductively isolated chromosomal taxa within #{A}n. funestus$. {W}hether the lack of support by mitochondrial {DNA} (mt{DNA}) and ribosomal {DNA} (r{DNA}) sequences is a result of the recent origin of the presumptive taxa, or of the absence of barriers to gene flow, remains to be elucidated, using more rapidly evolving markers such as microsatellites. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{MOUSTIQUE} ; {VECTEUR} ; {PALUDISME} ; {VARIABILITE} {GENETIQUE} ; {POLYMORPHISME} {GENETIQUE} ; {MARQUEUR} {GENETIQUE} ; {STRUCTURE} {DE} {POPULATION} ; {ADN} {RIBOSOMAL} ; {ADN} {MITOCHONDRIALI} ; {INVERSION} {CHROMOSOMIQUE} ; {SENEGAL} ; {BURKINA} {FASO} ; {KENYA}}, booktitle = {}, journal = {{M}olecular {E}cology}, volume = {8}, numero = {2}, pages = {289--297}, ISSN = {0962-1083}, year = {1999}, DOI = {10.1046/j.1365-294{X}.1999.00567.x}, URL = {https://www.documentation.ird.fr/hor/fdi:010017854}, }