@article{fdi:010017089, title = {{F}unctional analysis of the cassava vein mosaic virus promoter and its usage for cassava genetic engineering}, author = {{V}erdaguer, {B}. and {K}ochko, {A}lexandre de}, editor = {}, language = {{ENG}}, abstract = {{C}assava vein mosaic virus ({C}s{VMV}) is a plant pararetrovirus infecting cassava plants in {B}razil. {T}he promoter which directs the synthesis of the terminally redundant genome length viral ribonucleic acid ({RNA}), was isolated and used to express heterologous genes in transgenic plants. {A} deletion analysis of the upstream region of the promoter was carried out in order to study its functional structure. {E}ffects of the deletions were examined in tobacco plants using the vida reporter gene. {A}dditional data were obtained by protoplast transfection experiments and in vitro deoxyribonucleic acid ({DNA}) binding assays. {T}he constitutive pattern of the promoter expression is due to the promoter sequence of organo-specific cis elements. {T}he specifity of the virus for cassava plants and the constitutive as well as the molecular function of its promoter, make it a good alternative to the 35{S}. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{BIOTECHNOLOGIE} ; {GENIE} {GENETIQUE} ; {VIRUS} ; {TRANSFERT} {DE} {GENE} ; {CVMV}.{CASSAVA} {VEIN} {MOSAIC} {VIRUS} ; {PLANTE} {TRANSGENIQUE}}, booktitle = {}, journal = {{T}ropical {A}griculture}, volume = {75}, numero = {2}, pages = {317--319}, ISSN = {0041-3216}, year = {1998}, URL = {https://www.documentation.ird.fr/hor/fdi:010017089}, }