Gueye Mamadou auteur aut IRD James E.K. auteur aut IRD Kierans M. auteur aut IRD Sprent J.I. auteur aut IRD text journalArticle eng text/pdf reformatted digital access The infection of Vigna subterranea (formerly Voandzeia subterranea) by #Bradyrhizobium strain MAO 113 (isolated from V. subterranea) was examined by light and transmission electron microscopy. Bacteria accumulated on the epidermis close to root hairs, and subsequently entered the latter via infeciton threads. Most of the steps involved in nodule formation were generally characteristic of determinate nodules, such as those which form on the closely related #V. radiata. For example, nodule meristems were induced beneath the root epidermis adjacent to infected root hairs, but prior to infection of the meristem by rhizobia. Moreover, after the infection of some of the meristematic cells by the infection threads, and the release of the rhizobia into membrane-bound vesicles, the infection process ceased and dissemination of the rhizobia was division of already-infected host cells. However, there were some aspects of this process in V. subterranea which have been more commonly described in indeterminate nodules. These include long infection threads entering a number of cells within the meristems simultaneously and a matrix within infection threads which was strongly labelled with immunogold monoclonal antibodies, MAC236 and MAC265, which recognize epitopes on an intercellular glycoprotein. The MAC236 and MAC265 antibodies also recognized material in the unwalled infection droplets surrounding bacteria which were newly-released from the infection threads. The amount of labelling shown was more characteristic of the long infection threads seen in indeterminate nodules such as pea (Pisum sativum) and Neptunia plena... (D'après résumé d'auteur) specialized SYMBIOSE NODULE RACINAIRE LEGUMINEUSE A GRAINS BACTERIE INFESTATION MICROSCOPIE ELECTRONIQUE A TRANSMISSION 084SYMBIO01 14 177-184 1998 https://www.documentation.ird.fr/hor/fdi:010016794 [F B010016794] https://www.documentation.ird.fr/hor/fdi:010016794 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_53-54/010016794.pdf IRD - Base Horizon / Pleins textes 1999-02-01 2025-02-25 fdi:010016794 fre