%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Gueye, Mamadou
%A James, E.K.
%A Kierans, M.
%A Sprent, J.I.
%T The development and structure of root nodules on bambara groundnut [Voandzeia (Vigna) subterranea]
%D 1998
%L fdi:010016794
%G ENG
%J World Journal of Microbiology & Biotechnology
%K SYMBIOSE ; NODULE RACINAIRE ; LEGUMINEUSE A GRAINS ; BACTERIE ; INFESTATION ; MICROSCOPIE ELECTRONIQUE A TRANSMISSION
%P 177-184
%U https://www.documentation.ird.fr/hor/fdi:010016794
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_53-54/010016794.pdf
%V 14
%W Horizon (IRD)
%X The infection of #Vigna subterranea$ (formerly #Voandzeia subterranea) by #Bradyrhizobium$ strain MAO 113 (isolated from #V. subterranea) was examined by light and transmission electron microscopy. Bacteria accumulated on the epidermis close to root hairs, and subsequently entered the latter via infeciton threads. Most of the steps involved in nodule formation were generally characteristic of determinate nodules, such as those which form on the closely related #V. radiata$. For example, nodule meristems were induced beneath the root epidermis adjacent to infected root hairs, but prior to infection of the meristem by rhizobia. Moreover, after the infection of some of the meristematic cells by the infection threads, and the release of the rhizobia into membrane-bound vesicles, the infection process ceased and dissemination of the rhizobia was division of already-infected host cells. However, there were some aspects of this process in #V. subterranea$ which have been more commonly described in indeterminate nodules. These include long infection threads entering a number of cells within the meristems simultaneously and a matrix within infection threads which was strongly labelled with immunogold monoclonal antibodies, MAC236 and MAC265, which recognize epitopes on an intercellular glycoprotein. The MAC236 and MAC265 antibodies also recognized material in the unwalled infection droplets surrounding bacteria which were newly-released from the infection threads. The amount of labelling shown was more characteristic of the long infection threads seen in indeterminate nodules such as pea (#Pisum sativum$) and #Neptunia plena$... (D'après résumé d'auteur)
%$ 084SYMBIO01