@article{fdi:010016663,
  title = {{A}ntibodies and reactive {T} cells against the malaria heat-shock protein {P}f72/{H}sp70-1 and derived peptides in individuals continuously exposed to {P}lasmodium falciparum},
  author = {{B}ehr, {C}. and {S}arthou, {J}.{L}. and {R}ogier, {C}. and {T}rape, {J}ean-{F}ran{\c{c}}ois and {D}at, {M}.{H}.{Q}. and {M}ichel, {J}.{C}. and {A}ribot, {G}. and {D}ieye, {A}. and {C}laverie, {J}.{M}. and {D}ruihle, {P}. and {D}ubois, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}f72/{H}sp70-1, a heat-shock protein of m.w. 72 k{D}a from {P}lasmodium falciparum is one of the {A}g of interest to be included in a polyvalent vaccine against malaria. {I}t is one of the major immunogens present in a fraction of purified blood stage parasites that elicited protection against experimental infection of {S}aimiri monkeys with blood stages of {P}. falciparum. {I}t is present at all blood stages and one of its {B} cell epitopes is also detected on the surface of the infected hepatocyte. {M}oreover, {P}f72 appears to be well conserved among different isolates of {P}. falciparum. {W}e have examined the immune response against {P}f72/{H}sp70-1 in individuals from different age groups living in a holoendemic area ({W}est {A}frica). {T}he immune response against the native {A}g (purified from schizonts and called {P}f/{H}sp70) was analyzed both at the humoral level by {ELISA} and at the cellular level by assessing in vitro proliferation and {IFN}-gamma production of {PBMC}. {O}f the individuals studied 52% had a statistically significant level of anti-{P}f/{H}sp70 antibodies as compared with unexposed individuals. {T}hese positive individuals showed a heterogeneous distribution because significant levels of antibodies were found in 70% of the adults but in only 26% of the children. {T}he presence of {P}f/{H}sp70-specific reactive {T} cells in the blood was detected in 32% of the individuals. {T}he total anti-{P}f/{H}sp70 antibody level ({I}g{G}+{I}g{M}) appeared strongly age related and correlated positively with parasite exposure, whereas the {T} cell response failed to correlate either with the antibody level or with age. {M}oreover, {PBMC} of donors responded to the {P}f/{H}sp70 in a dissociated way, namely, by either {T} cell proliferation or {IFN}-gamma production. {T}en synthetic peptides based on sequences found in the {C}-terminal part of {P}f72/{H}sp70-1 were further tested as potential {T} cell epitopes. {T}he proliferative response of {PBMC} from individuals continuously exposed to the parasite showed that three peptides more frequently trigger significant {T} cell proliferation (in 21% to 27% of the individuals) and three others less frequently (10%). {N}one of these peptides allowed detection of reactive {T} cells in {PBMC} of {E}uropeans with no previous exposure to malaria. {S}ome of the stimulating peptides are highly similar to human heat-shock {H}sc and {H}sp70 with large stretches of identical amino acids.},
  keywords = {{PALUDISME} ; {IMMUNOLOGIE} ; {TRANSMISSION} ; {POU} ; {ANTICORPS} ; {ANTIGENE} ; {ZONE} {HOLOENDEMIQUE} ; {LYMPHOCYTE} {T} ; {PF}72/{HSP}70-1 ; {SENEGAL} ; {SINE} {SALOUM} ; {FATICK} {REGION} ; {DIELMO}},
  booktitle = {},
  journal = {{J}ournal of {I}mmunology},
  volume = {149},
  numero = {10},
  pages = {3321--3330},
  ISSN = {0022-1767},
  year = {1992},
  DOI = {10.4049/jimmunol.149.10.3321},
  URL = {https://www.documentation.ird.fr/hor/fdi:010016663},
}