@article{fdi:010016659,
  title = {{I}mmune response in mouse and malaria-exposed humans to peptides derived from {P}f11-1, a highly repetitive megadalton protein of {P}lasmodium falciparum},
  author = {{S}cherf, {A}. and {B}ehr, {C}. and {S}arthou, {J}.{L}. and {P}la, {M}. and {R}ogier, {C}. and {T}rape, {J}ean-{F}ran{\c{c}}ois and {P}ereira da {S}ilva, {L}. and {D}ubois, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e have investigated the immune response against the {P}lasmodium falciparum gametocyte-specific antigen {P}f11-1. {T}his megadalton parasite molecule has been implicated in the process of erythrocyte rupture during gametogenesis. {T}he molecule is composed in great part of degenerated nonapeptide motifs which are tandemly repeated several hundred times. {A} computer algorithm searching for {T} sites predicted that the entire repeat region of the {P}f11-1 represents potential {T} cell antigenic major histocompatibility complex class {II}-binding sites. {T}o test this hypothesis, synthetic peptides corresponding to two nonamer subtype repeats, differing only at two amino acid positions, were used to immunize congenic mouse strains. {B}oth peptides were shown to contain both {B} and {T} cell epitopes. {T}he immune response is restricted to the {H}-2d and {H}-2k haplotypes. {T}he {T} cell response against the peptides appeared to be highly specific, clearly discriminating between the two similar nonamer repeat sequences, whereas the humoral response produced cross-reacting antibodies. {W}e also investigated the humoral and {T} cell reactivities of {P} falciparum-primed individuals in {W}est {A}frica against the synthetic {P}f11-1 peptides. {A}mong 51 individuals 35 had antibodies to at least one of the two peptides and a majority of them (28) had antibodies reacting with both peptides. {T}he cellular response was analyzed by [{H}-3] thymidine incorporation or interferon-gamma release. {T}here was considerable variation in the response to the two peptides. {A}mong the human samples 36% responded to one repeat subtype, while only 13% responded to the second subtype. {I}nterestingly, in individual donors the {T} cell response to both peptides are associated, suggesting that, as shown for mice, the response is restricted by a genetic element. {T}he data obtained on the two subtypes of the nonamer repeat region suggest that the entire {P}f11-1 molecule might induce an unusually heterogenous {B} and {T} cell response during natural infection in man.},
  keywords = {{PALUDISME} ; {IMMUNOLOGIE} ; {ANTIGENE} ; {GAMETE} ; {LYMPHOCYTE} ; {GAMETOCYTE} ; {REACTION} {CROISEE} ; {SENEGAL}},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {I}mmunology},
  volume = {23},
  numero = {},
  pages = {1574--1581},
  ISSN = {0014-2980},
  year = {1993},
  DOI = {10.1002/eji.1830230727},
  URL = {https://www.documentation.ird.fr/hor/fdi:010016659},
}