@article{fdi:010015961,
  title = {{P}otency of products based on {B}acillus thuringiensis var. israelensis : interlaboratory variations},
  author = {{S}kovmand, {O}le and {T}hiery, {I}. and {B}enzon, {G}.{L}. and {S}in{\`e}gre, {G}. and {M}onteny, {N}icole and {B}ecker, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ix quality-control laboratories in 4 countries independently bioassayed aliquots of a flowable formulation of #{B}acillus thuringiensis$ var. #israelensis$ ({B}.t.i.) against the international standard powder {IPS}-82. {A}ll laboratories substantially followed {W}orld {H}ealth {O}rganization or {U}.{S}. {D}epartment of {A}griculture standard protocols. {S}ignificant differences were found in resulting potency values between laboratories. {F}actors that may have influenced results, such as age, stage, and strain of larvae used, amount and type of food provided to larvae, and processing of samples were examined. {U}se of different rearing temperatures, different strains of #{A}edes aegypti$ {L}., or late 3rd instars vs. the recommended early 4th instars did not explain the inconsistencies. {T}he slope of the dose-response curve of the {IPS}-82 powder was influenced by particle size, which varied with the nature and duration of sample homogenization. {L}aboratories using low-intensity processing obtained a greater slope in the dose-response curve for the flowable product than for the powder standard. {T}he type and quantity of food provided to larvae affected susceptibility. {L}arvae fed an excess of protein-rich food became 4th instars in 3 days and were less susceptible to {B}.t.i. than those fed smaller quantities of carbohydrate-rich food that became 4th instars in 5-7 days. {O}verall, deviations from standard protocols with regard to larval stage, holding temperature, and lighting regime may not be as important as differences in sample processing and pretest rearing conditions. {T}he need to improve standardization in these areas, which are not clearly specified in current protocols, is discussed. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{INSECTICIDE} {BIOLOGIQUE} ; {LARVE} ; {LUTTE} {BIOLOGIQUE} ; {BACTERIE} ; {POUVOIR} {PATHOGENE} ; {TEST} {DE} {SENSIBILITE} ; {EXPERIMENTATION} {EN} {LABORATOIRE}},
  booktitle = {},
  journal = {{J}ournal of the {A}merican {M}osquito {C}ontrol {A}ssociation},
  volume = {14},
  numero = {3},
  pages = {298--304},
  ISSN = {8756-971{X}},
  year = {1998},
  URL = {https://www.documentation.ird.fr/hor/fdi:010015961},
}