Nod factors thin-layer chromatography profiling as a tool to characterize symbiotic specificity of rhizobial strains : application to #Sinorhizobium saheli$, #S. teranga$, and #Rhizobium$ sp. strains isolated from #Acacia$ and #Sesbania$

Nod factors thin-layer chromatography profiling as a tool to characterize symbiotic specificity of rhizobial strains : application to #Sinorhizobium saheli$, #S. teranga$, and #Rhizobium$ sp. strains isolated from #Acacia$ and #Sesbania$ Lortet Gilles auteur aut IRD Méar Nathalie auteur aut IRD Lorquin Jean auteur aut IRD Dreyfus Bernard auteur aut IRD Lajudie Philippe de auteur aut IRD Rosenberg C. auteur aut IRD Boivin Catherine auteur aut IRD text journalArticle eng text/pdf reformatted digital access Rhizobia isolated from Acacia or Sesbania belong to several taxonomic groups, including the newly described species Sinorhizobium saheli, Sinorhizobium teranga, and the so-called cluster U. A collection of strains belonging to these different groups was analyzed in order to determine whether the host range of a strain could be correlated with various molecular nodulation determinants. Nodulation tests showed that, independently of their taxonomic position, all the strains isolated from the same plant genus exhibited a similar host range, which was different for Sesbania and Acacia isolates. The fact that S. teranga strains nodulate either Acacia or Sesbania led us to subdivide this species into biovars acaciae and sesbaniae. Thin-layer chromatography (TLC) analysis of the Nod factors synthesized by overproducing strains showed that strains isolated from the same plant genus exhibited similar TLC profiles and profiles of Acacia and Sesbania symbionts were easily distinguishable, Acacia strains producing, in particular, sulfated molecules. In contrast, no correlation could be established between the host range of a strain and its plasmid content, the nature of the nod gene inducers or the presence of DNA sequences homologous to specific nod genes. We thus propose that Nod factor TLC profiling may be used as an easy and powerful tool for the classification of rhizobial strains on the basis of their symbiotic properties. (Résumé d'auteur) specialized NODULE RACINAIRE FIXATION BIOLOGIQUE DE L'AZOTE PLANTE HOTE BACTERIE SOUCHE GENOME HYBRIDATION CHROMATOGRAPHIE 084SYMBIO02 Molecular Plant-Microbe Interactions 9 8 736-747 1996 0894-0282 https://www.documentation.ird.fr/hor/fdi:010015493 0894-0282 [F B010015493] https://www.documentation.ird.fr/hor/fdi:010015493 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_51-52/010015493.pdf IRD - Base Horizon / Pleins textes 1998-07-02 2018-06-15 fdi:010015493 fre