%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Loussert-Ajaka, I.
%A Menu, E.
%A Apetrei, C.
%A Peeters, Martine
%A Damond, F.
%A Mauclère, P.
%A Eberle, J.
%A Brengues, Cécile
%A Saragosti, S.
%A Barré-Sinoussi, F.
%A Brun-Vezinet, F.
%A Simon, F.
%T HIV type 1 diversity and the reliability of the heteroduplex mobility assay
%D 1998
%L fdi:010014699
%G ENG
%J AIDS Research and Human Retroviruses
%@ 0889-2229
%K SIDA ; VIRUS ; DIVERSITE GENETIQUE ; METHODE D'ANALYSE ; TECHNIQUE PCR ; PHYLOGENIE
%K VIH-1
%N 10
%P 877-883
%R 10.1089/aid.1998.14.877
%U https://www.documentation.ird.fr/hor/fdi:010014699
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_51-52/010014699.pdf
%V 14
%W Horizon (IRD)
%X We investigated HIV-1 diversity by means of heteroduplex mobility assay (HMA) genotyping. We studied 199 samples from patients originating from 26 countries and living in France. The HMA successfully genotyped 182 (91 %) of these samples, as follows : 77 (42 %) subtype A, 57 (31 %) subtype B, 5 (3 %) subtype C, 5 (3 %) subtype D, 8 (4 %) subtype E, 22 (12 %) subtype F, 5 (3 %) subtype G, and 3 (2 %) subtype H. We were not able to genotype 12 samples by means of the HMA. These latter strains were sequenced, and phylogenetic analyses revealed that they were highly divergent subtype A-, D-, or G-related strains. Eight (of 12) subtype D strains were indeterminate by HMA, owing to the broad intrasubtype diversity, suggesting that new reference subtype D plasmids are required, as previously proposed. Thirty-seven strains belonging to the different subtypes were sequenced, and the results showed perfect concordance with the HMA results. Interlaboratory quality controls confirmed the reliability of the HMA for HIV-1 subtyping, despite the extensive viral variability. However, plasmid selection must be continuously revised to cover viral diversification. (Résumé d'auteur)
%$ 052MALTRA03