@article{fdi:010014699,
  title = {{HIV} type 1 diversity and the reliability of the heteroduplex mobility assay},
  author = {{L}oussert-{A}jaka, {I}. and {M}enu, {E}. and {A}petrei, {C}. and {P}eeters, {M}artine and {D}amond, {F}. and {M}aucl{\`e}re, {P}. and {E}berle, {J}. and {B}rengues, {C}{\'e}cile and {S}aragosti, {S}. and {B}arr{\'e}-{S}inoussi, {F}. and {B}run-{V}ezinet, {F}. and {S}imon, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e investigated {HIV}-1 diversity by means of heteroduplex mobility assay ({HMA}) genotyping. {W}e studied 199 samples from patients originating from 26 countries and living in {F}rance. {T}he {HMA} successfully genotyped 182 (91 %) of these samples, as follows : 77 (42 %) subtype {A}, 57 (31 %) subtype {B}, 5 (3 %) subtype {C}, 5 (3 %) subtype {D}, 8 (4 %) subtype {E}, 22 (12 %) subtype {F}, 5 (3 %) subtype {G}, and 3 (2 %) subtype {H}. {W}e were not able to genotype 12 samples by means of the {HMA}. {T}hese latter strains were sequenced, and phylogenetic analyses revealed that they were highly divergent subtype {A}-, {D}-, or {G}-related strains. {E}ight (of 12) subtype {D} strains were indeterminate by {HMA}, owing to the broad intrasubtype diversity, suggesting that new reference subtype {D} plasmids are required, as previously proposed. {T}hirty-seven strains belonging to the different subtypes were sequenced, and the results showed perfect concordance with the {HMA} results. {I}nterlaboratory quality controls confirmed the reliability of the {HMA} for {HIV}-1 subtyping, despite the extensive viral variability. {H}owever, plasmid selection must be continuously revised to cover viral diversification. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{SIDA} ; {VIRUS} ; {DIVERSITE} {GENETIQUE} ; {METHODE} {D}'{ANALYSE} ; {TECHNIQUE} {PCR} ; {PHYLOGENIE} ; {VIH}-1},
  booktitle = {},
  journal = {{AIDS} {R}esearch and {H}uman {R}etroviruses},
  volume = {14},
  numero = {10},
  pages = {877--883},
  ISSN = {0889-2229},
  year = {1998},
  DOI = {10.1089/aid.1998.14.877},
  URL = {https://www.documentation.ird.fr/hor/fdi:010014699},
}