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    <titleInfo>
      <title>Acetamide degradation by a continuous-fed batch culture of #Bacillus sphaericus$</title>
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    <name type="personnal">
      <namePart type="family">Ramirez</namePart>
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    <name type="personnal">
      <namePart type="family">Monroy</namePart>
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    <name type="personnal">
      <namePart type="family">Favela</namePart>
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    <name type="personnal">
      <namePart type="family">Guyot</namePart>
      <namePart type="given">Jean-Pierre</namePart>
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    <name type="personnal">
      <namePart type="family">Cruz</namePart>
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    <abstract>The methanogenesis of acetamide occurs through a two-step reaction in methanogenic sludges. First, acetamide is hydrolyzed to acetate and ammonia by a strict aerobic bacterium (Bacillus sphaericus), then acetate is used by Bacillus as carbon source or converted to methane by methanogens. In this work, the kinetics of acetamide degradation by B. sphaericus was studied in a continuous reactor with biomass accumulation, fed with acetamide. The oxygen supplied was dissolved in the feed (6.4 mg/L) to resemble conditions in an anaerobic wastewater treatment reactor. A reaction in series model (acetamide -&gt; acetate -&gt; biomass) was used to find the kinetic parameters. Results show that B. sphaericus can hydrolyze acetamide in a second-order reaction with K1 = 1.1 L/g/d, implying that the amount of biomass determines the rate and that no reaction will take place at specific loading rates greater than 35 gAm/gX/d. (Résumé d'auteur)</abstract>
    <targetAudience authority="marctarget">specialized</targetAudience>
    <subject authority="local">
      <topic>FERMENTATION</topic>
      <topic>ANAEROBIOSE</topic>
      <topic>METHANOGENESE</topic>
      <topic>BACTERIE</topic>
      <topic>MILIEU DE CULTURE</topic>
      <topic>HYDROLYSE</topic>
      <topic>BIOMASSE</topic>
    </subject>
    <subject>
      <topic>ACETAMIDE</topic>
    </subject>
    <classification authority="local">084FERMEN02</classification>
    <relatedItem type="host">
      <titleInfo>
        <title>Biotechnology for fuels and chemicals</title>
      </titleInfo>
      <name type="personnal">
        <namePart type="family">Finkelstein</namePart>
        <namePart type="given">M.</namePart>
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        <affiliation>IRD</affiliation>
      </name>
      <name type="personnal">
        <namePart type="family">Davison</namePart>
        <namePart type="given">B.H.</namePart>
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        <affiliation>IRD</affiliation>
      </name>
      <part>
        <detail type="volume">
          <number>70-72</number>
        </detail>
        <extent unit="pages">
          <list> 215-223</list>
        </extent>
      </part>
      <originInfo>
        <dateIssued key="date">1998</dateIssued>
      </originInfo>
      <name type="conference">
        <namePart>Biotechnology for Fuels and Chemicals : Symposium, 9., Colorado Springs (USA), 1997/05/04-08</namePart>
      </name>
    </relatedItem>
    <identifier type="uri">https://www.documentation.ird.fr/hor/fdi:010014123</identifier>
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      <recordCreationDate encoding="w3cdtf">1998-07-16</recordCreationDate>
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