Horizon 1 1 5 OS CH : Chapitres d'ouvrages scientifiques Biotechnology for fuels and chemicals Applied Biochemistry and Biotechnology Biotechnology for Fuels and Chemicals : Symposium 215-223 FERMENTATION ANAEROBIOSE METHANOGENESE BACTERIE MILIEU DE CULTURE HYDROLYSE BIOMASSE ACETAMIDE 1998 1997/05/04-08 fdi:010014123 ENG Applied Biochemistry and Biotechnology 0273-2289 https://www.documentation.ird.fr/hor/fdi:010014123 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010014123.pdf 70-72 Horizon (IRD) The methanogenesis of acetamide occurs through a two-step reaction in methanogenic sludges. First, acetamide is hydrolyzed to acetate and ammonia by a strict aerobic bacterium (#Bacillus sphaericus$), then acetate is used by #Bacillus$ as carbon source or converted to methane by methanogens. In this work, the kinetics of acetamide degradation by #B. sphaericus$ was studied in a continuous reactor with biomass accumulation, fed with acetamide. The oxygen supplied was dissolved in the feed (6.4 mg/L) to resemble conditions in an anaerobic wastewater treatment reactor. A reaction in series model (acetamide -> acetate -> biomass) was used to find the kinetic parameters. Results show that #B. sphaericus$ can hydrolyze acetamide in a second-order reaction with K1 = 1.1 L/g/d, implying that the amount of biomass determines the rate and that no reaction will take place at specific loading rates greater than 35 gAm/gX/d. (Résumé d'auteur) 084FERMEN02