%0 Book Section
%9 OS CH : Chapitres d'ouvrages scientifiques
%A Ramirez, F.
%A Monroy, O.
%A Favela, E.
%A Guyot, Jean-Pierre
%A Cruz, F.
%T Acetamide degradation by a continuous-fed batch culture of Bacillus sphaericus
%B Biotechnology for fuels and chemicals
%D 1998
%E Finkelstein, M.
%E Davison, B.H.
%L fdi:010014123
%G ENG
%J Applied Biochemistry and Biotechnology
%@ 0273-2289
%K FERMENTATION ; ANAEROBIOSE ; METHANOGENESE ; BACTERIE ; MILIEU DE CULTURE ; HYDROLYSE ; BIOMASSE
%K ACETAMIDE
%P 215-223
%U https://www.documentation.ird.fr/hor/fdi:010014123
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010014123.pdf
%V 70-72
%W Horizon (IRD)
%X The methanogenesis of acetamide occurs through a two-step reaction in methanogenic sludges. First, acetamide is hydrolyzed to acetate and ammonia by a strict aerobic bacterium (#Bacillus sphaericus$), then acetate is used by #Bacillus$ as carbon source or converted to methane by methanogens. In this work, the kinetics of acetamide degradation by #B. sphaericus$ was studied in a continuous reactor with biomass accumulation, fed with acetamide. The oxygen supplied was dissolved in the feed (6.4 mg/L) to resemble conditions in an anaerobic wastewater treatment reactor. A reaction in series model (acetamide -> acetate -> biomass) was used to find the kinetic parameters. Results show that #B. sphaericus$ can hydrolyze acetamide in a second-order reaction with K1 = 1.1 L/g/d, implying that the amount of biomass determines the rate and that no reaction will take place at specific loading rates greater than 35 gAm/gX/d. (Résumé d'auteur)
%B Biotechnology for Fuels and Chemicals : Symposium
%8 1997/05/04-08
%$ 084FERMEN02