@article{fdi:010013974,
  title = {{C}ryopreservation of sugarcane embryogenic callus using a simplified freezing process},
  author = {{M}artinez-{M}ontero, {M}.{E}. and {G}onzalez-{A}rnao, {M}.{T}. and {B}orroto-{N}ordelo, {C}. and {P}uentes-{D}iaz, {C}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{A} simplified freezing process was developed and successfully applied to embryogenic calluses of three sugarcane commercial hybrids (#{S}accharum$ sp. cv. {CP} 5243, {C} 91-301 and {C} 1051-73). {T}o obtain optimal survival, the calluses were pretreated with a cryoprotective solution containing 10% {DMSO} and 0.3 to 0.75 {M} sucrose. {F}or freezing, the samples were immersed in an alcohol bath placed in a -40°{C} freezer, thus allowing a freezing rate comprised between 0.4 and 0.6°{C}/min. {S}amples were held at that temperature for 2 h before immersion in liquid nitrogen. {T}he highest survival rates of cryopreserved calluses ranged between 20 and 94% depending on the variety, and fully developed plantlets could be obtained from regenerating calluses. {E}mbryogenic calluses of one variety were stored for 14 months in liquid nitrogen without any effect on their survival rate and plantlet production. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{CULTURE} {IN} {VITRO} ; {CAL} ; {CANNE} {A} {SUCRE} ; {CRYOCONSERVATION} ; {MILIEU} {DE} {CULTURE}},
  booktitle = {},
  journal = {{C}ryo-{L}etters},
  volume = {19},
  numero = {3},
  pages = {171--176},
  ISSN = {0143-2044},
  year = {1998},
  URL = {https://www.documentation.ird.fr/hor/fdi:010013974},
}