@article{fdi:010013583,
  title = {{M}ovement of rice yellow mottle virus between xylem cells through pit membranes},
  author = {{O}palka, {N}. and {B}rugidou, {C}hristophe and {B}onneau, {C}. and {N}icole, {M}ichel and {B}eachy, {R}.{N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he translocation of rice yellow mottle virus ({RYMV}) within tissues of inoculated and systematically infected #{O}ryza sativa$ {L}. leaves was characterized by {W}estern immunoblotting, {N}orthern blotting, and electron microscopy of thin sections. {I}n inoculated leaves, {RYMV} {RNA} and coat protein first were detected at 3 and 5 days postinoculation, respectively. {B}y six days postinoculation, {RYMV} had spread systematically to leaves, and virus particles were observed in most cell types, including epidermal, mesophyll, bundle sheath, and vascular parenchyma cells. {M}ost of the virions accumulated in large crystalline patches in xylem parenchyma cells and sieve elements. {C}olocalization of a cell wall marker for cellulosic beta-(1-4)-{D}-glucans and anti-{RYMV} antibodies over vessel pit membranes suggests a pathway for virus migration between vessels. {W}e propose that the partial digestion of pit membranes resulting from programmed cell death may permit virus migration through them, concomitant with autolysis. {I}n addition, displacement of the {C}a2+ from pit membranes to virus particles may contribute to the disruption of the pit membranes and facilitate systemic virus transport. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{PATHOLOGIE} {VEGETALE} ; {RIZ} ; {VIROSE} ; {FEUILLE} ; {INOCULATION} ; {RELATION} {HOTE} {PARASITE} ; {MICROSCOPIE} {ELECTRONIQUE} ; {IMMUNOLOGIE}},
  booktitle = {},
  journal = {{P}roceedings of the {N}ational {A}cademy of {S}ciences},
  volume = {95},
  numero = {6},
  pages = {3323--3328},
  ISSN = {0027-8424},
  year = {1998},
  DOI = {10.1073/pnas.95.6.3323},
  URL = {https://www.documentation.ird.fr/hor/fdi:010013583},
}