Bibollet-Ruche F. auteur aut IRD Cuny Gérard auteur aut IRD Pourrut Xavier auteur aut IRD Brengues Cécile auteur aut IRD Galat-Luong Anh auteur aut IRD Galat Gérard auteur aut IRD Delaporte Eric auteur aut IRD text journalArticle eng text/pdf reformatted digital access We have characterized the spliced transcripts of nef and envelope genes of SIVagm from African green monkey of the sabaeus subspecies. Most of the transcripts we have studied, representing the most abundant mRNA species in our assay, have undergone a specific splicing event that removes a part of the trans-activation response (TAR) element. This region is predicted to form a stable secondary structure (four stem-loop elements in SIVagm-sab) that affects the trans-activation of viral gene expression by Tat and the translation of the viral transcripts. Contrary to what is observed in other viruses, in which this R-region splicing has also been described (e.g. , HIV-2), the LTR splicing in SIVagm-sab removes part of the first stem-loop and the following ones, nearly completely disrupting the TAR element secondary structure. Because LTR splicing seems to be a conserved feature among the strains we have characterized, these results suggest that this phenomenon could have important consequences for virus replication, pathogenicity, and latency. (Résumé d'auteur) specialized VIRUS POLYMORPHISME GENETIQUE MUTATION SIV.VIRUS IMMUNODEFICIENCE SIMIENNE EXPRESSION GENETIQUE PATHOGENIE SINGE VERT AFRIQUE DE L'OUEST 052MALTRA03 14 6 515-519 1998 0889-2229 https://www.documentation.ird.fr/hor/fdi:010013581 10.1089/aid.1998.14.515 0889-2229 [F B010013581] https://www.documentation.ird.fr/hor/fdi:010013581 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010013581.pdf IRD - Base Horizon / Pleins textes 1998-05-07 2020-08-03 fdi:010013581 fre