Horizon 1 1 17 ACLN : Articles dans des revues avec comité de lecture non répertoriées par l'AERES AIDS Research and Human Retroviruses 515-519 VIRUS POLYMORPHISME GENETIQUE MUTATION SIV.VIRUS IMMUNODEFICIENCE SIMIENNE EXPRESSION GENETIQUE PATHOGENIE SINGE VERT AFRIQUE DE L'OUEST 1998 fdi:010013581 ENG AIDS Research and Human Retroviruses 0889-2229 6 10.1089/aid.1998.14.515 https://www.documentation.ird.fr/hor/fdi:010013581 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010013581.pdf 14 Horizon (IRD) We have characterized the spliced transcripts of nef and envelope genes of SIVagm from African green monkey of the #sabaeus$ subspecies. Most of the transcripts we have studied, representing the most abundant mRNA species in our assay, have undergone a specific splicing event that removes a part of the trans-activation response (TAR) element. This region is predicted to form a stable secondary structure (four stem-loop elements in SIVagm-sab) that affects the trans-activation of viral gene expression by Tat and the translation of the viral transcripts. Contrary to what is observed in other viruses, in which this R-region splicing has also been described (e.g. , HIV-2), the LTR splicing in SIVagm-sab removes part of the first stem-loop and the following ones, nearly completely disrupting the TAR element secondary structure. Because LTR splicing seems to be a conserved feature among the strains we have characterized, these results suggest that this phenomenon could have important consequences for virus replication, pathogenicity, and latency. (Résumé d'auteur) 052MALTRA03