%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Dussert, Stéphane
%A Chabrillange, Nathalie
%A Engelmann, Florent
%A Anthony, François
%A Louarn, Jacques
%A Hamon, Serge
%T Cryopreservation of seeds of four coffee species (Coffea arabica, C. costatifructa, C. racemosa and C. sessiliflora) : importance of water content and cooling rate
%D 1998
%L fdi:010013239
%G ENG
%J Seed Science Research
%@ 0960-2585
%K AMELIORATION DES PLANTES ; CONGELATION ; CRYOCONSERVATION ; GERMINATION ; RESSOURCES GENETIQUES ; DESSICCATION
%N 1
%P 9-15
%R 10.1017/S096025850000386X
%U https://www.documentation.ird.fr/hor/fdi:010013239
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_47-48/010013239.pdf
%V 8
%W Horizon (IRD)
%X In the range of the water contents studied (0.1-0.4 g H2O g/dw), #Coffea arabica$ seeds were less sensitive to desiccation than #C. costatifructa$, #C. racemosa$ and #C. sessiliflora$ seeds. At 0.20 g H2O g/dw, 53% of #C. arabica$ seeds germined after direct immersion in LN (rapid cooling, 200°C/min), but none of them developed into normal seedlings. By contrast, in #C. costatifructa$, #C. racemosa$ and #C. sessiliflora$, when seeds were dehydrated to the optimal water content (0.19, 0.28 and 0.31 g H2O g/dw, respectively), the percentages of seeds which developed into normal seedlings after LN exposure were 26, 78 and 31% of the desiccation control, respectively. Normal seedlings could be recovered from cryopreserved #C. arabica$ seeds only if they were desiccated to 0.20 g H2O g/dw and precooled slowly to -50°C prior to immersion in LN. Precooling seeds at 2°C/min allowed 25% of seeds to develop into normal seedlings. The thawing rate had no effect on the survival of cryopreserved #C. arabica$ seeds. In all cryopreservation experiments, the total germination did not reflect the percentage of seeds which developed into normal seedlings. Examination of excised embryos indicated a partial explanation of this difference since only the shoot apex was destroyed in abnormal embryos, whereas the hypocotyl and radicle were normal. (Résumé d'auteur)
%$ 076AMEPLA02