@article{fdi:010013239,
  title = {{C}ryopreservation of seeds of four coffee species ({C}offea arabica, {C}. costatifructa, {C}. racemosa and {C}. sessiliflora) : importance of water content and cooling rate},
  author = {{D}ussert, {S}t{\'e}phane and {C}habrillange, {N}athalie and {E}ngelmann, {F}lorent and {A}nthony, {F}ran{\c{c}}ois and {L}ouarn, {J}acques and {H}amon, {S}erge},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n the range of the water contents studied (0.1-0.4 g {H}2{O} g/dw), #{C}offea arabica$ seeds were less sensitive to desiccation than #{C}. costatifructa$, #{C}. racemosa$ and #{C}. sessiliflora$ seeds. {A}t 0.20 g {H}2{O} g/dw, 53% of #{C}. arabica$ seeds germined after direct immersion in {LN} (rapid cooling, 200°{C}/min), but none of them developed into normal seedlings. {B}y contrast, in #{C}. costatifructa$, #{C}. racemosa$ and #{C}. sessiliflora$, when seeds were dehydrated to the optimal water content (0.19, 0.28 and 0.31 g {H}2{O} g/dw, respectively), the percentages of seeds which developed into normal seedlings after {LN} exposure were 26, 78 and 31% of the desiccation control, respectively. {N}ormal seedlings could be recovered from cryopreserved #{C}. arabica$ seeds only if they were desiccated to 0.20 g {H}2{O} g/dw and precooled slowly to -50°{C} prior to immersion in {LN}. {P}recooling seeds at 2°{C}/min allowed 25% of seeds to develop into normal seedlings. {T}he thawing rate had no effect on the survival of cryopreserved #{C}. arabica$ seeds. {I}n all cryopreservation experiments, the total germination did not reflect the percentage of seeds which developed into normal seedlings. {E}xamination of excised embryos indicated a partial explanation of this difference since only the shoot apex was destroyed in abnormal embryos, whereas the hypocotyl and radicle were normal. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{AMELIORATION} {DES} {PLANTES} ; {CONGELATION} ; {CRYOCONSERVATION} ; {GERMINATION} ; {RESSOURCES} {GENETIQUES} ; {DESSICCATION}},
  booktitle = {},
  journal = {{S}eed {S}cience {R}esearch},
  volume = {8},
  numero = {1},
  pages = {9--15},
  ISSN = {0960-2585},
  year = {1998},
  DOI = {10.1017/{S}096025850000386{X}},
  URL = {https://www.documentation.ird.fr/hor/fdi:010013239},
}