@article{fdi:010012614,
  title = {{D}etection of the {C}assava bacterial blight pathogen, {X}anthomonas axonopodis pv. manihotis, by polymerase chain reaction},
  author = {{V}erdier, {V}al{\'e}rie and {M}osquera, {G}. and {A}ssigbets{\'e}, {K}omi},
  editor = {},
  language = {{ENG}},
  abstract = {{C}assava bacterial blight, caused by #{X}anthomonas axonopodis$ pv. #manihotis$, is of significant concern wherever cassava is grown. {T}he movement of infected, asymptomatic stems is a major means of pathogen dispersal. {A} reliable and sensitive diagnostic procedure is necessary for the safe movement of cassava planting material. {W}e used a cloned and sequenced pathogenicity gene of #{X}. axonopodis$ pv. #manihotis$ to develop a polymerase chain reaction ({PCR}) test for this pathogen. {A} set of primers directed the amplification of an 898-bp fragment in all 107 pathogenic strains of #{X}. axonopodis$ pv. #manihotis$ tested. {PCR} products were not observed when genomic {DNA} was tested for 27 strains of other xanthomonads, for saprophytic bacteria, or for five nonpathogenic strains of #{X}. axonopodis$ pv. #manihotis$. {T}he primers worked well for pathogen detection in direct {PCR} assays of #{X}. axonopodis$ pv. #manihotis$ colonies grown on liquid medium and in {PCR} assays of extracts from leaf and stem lesions. {T}he minimum number of cells that could be detected from cassava stem and leaf lesions was 3 {X} 100 to 10000 {CFU}/ml. {T}he {PCR} assays proved to be relatively sensitive and could become very useful in detecting the pathogen in cassava planting material. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{PATHOLOGIE} {VEGETALE} ; {BACTERIOSE} ; {AGENT} {PATHOGENE} ; {IDENTIFICATION} ; {TECHNIQUE} {PCR}},
  booktitle = {},
  journal = {{P}lant {D}isease},
  volume = {82},
  numero = {1},
  pages = {79--83},
  ISSN = {0191-2917},
  year = {1998},
  DOI = {10.1094/{PDIS}.1998.82.1.79},
  URL = {https://www.documentation.ird.fr/hor/fdi:010012614},
}