%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Léry, Xavier
%A Abol-Ela, S.
%A Giannotti, J.
%T In vitro isolation of clones from the tunisian isolate of the potato tuber moth (Phthorimaea operculella) granulosis virus
%D 1997
%L fdi:010012233
%G ENG
%J Bulletin of Faculty of Agriculture - University of Cairo
%@ 0526-8613
%K MALADIE DES PLANTES ; VIRUS ; POMME DE TERRE ; CLONE ; CULTURE IN VITRO ; VARIABILITE GENETIQUE ; ADN ; METHODE D'ANALYSE
%K GRANULOSIS VIRUS
%N 3
%P 585-596
%U https://www.documentation.ird.fr/hor/fdi:010012233
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_47-48/010012233.pdf
%V 48
%W Horizon (IRD)
%X Clones were obtained in vitro, as a first record, from the Tunisian isolate of the potato tuber moth granulosis virus (PTM GV). Using three successive selections with plaque agarose overlay, 12 clones were established. Among them, five were selected for their better in vitro multiplication after being tested with a specific DNA probe and amplified both in vitro and in vivo. Restriction endonuclease analysis of the DNA extract demonstrated minor genotype differences between the clones, indicating the genetic heterogeneity of the viral population of the Tunisian isolate of PTMGV. (Résumé d'auteur)
%$ 076MALPLA