@article{fdi:010012233, title = {{I}n vitro isolation of clones from the tunisian isolate of the potato tuber moth ({P}hthorimaea operculella) granulosis virus}, author = {{L}{\'e}ry, {X}avier and {A}bol-{E}la, {S}. and {G}iannotti, {J}.}, editor = {}, language = {{ENG}}, abstract = {{C}lones were obtained in vitro, as a first record, from the {T}unisian isolate of the potato tuber moth granulosis virus ({PTM} {GV}). {U}sing three successive selections with plaque agarose overlay, 12 clones were established. {A}mong them, five were selected for their better in vitro multiplication after being tested with a specific {DNA} probe and amplified both in vitro and in vivo. {R}estriction endonuclease analysis of the {DNA} extract demonstrated minor genotype differences between the clones, indicating the genetic heterogeneity of the viral population of the {T}unisian isolate of {PTMGV}. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{MALADIE} {DES} {PLANTES} ; {VIRUS} ; {POMME} {DE} {TERRE} ; {CLONE} ; {CULTURE} {IN} {VITRO} ; {VARIABILITE} {GENETIQUE} ; {ADN} ; {METHODE} {D}'{ANALYSE} ; {GRANULOSIS} {VIRUS}}, booktitle = {}, journal = {{B}ulletin of {F}aculty of {A}griculture - {U}niversity of {C}airo}, volume = {48}, numero = {3}, pages = {585--596}, ISSN = {0526-8613}, year = {1997}, URL = {https://www.documentation.ird.fr/hor/fdi:010012233}, }