%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES %A Thiery, I. %A Baldet, Thierry %A Barbazan, Philippe %A Becker, N. %A Junginger, B. %A Mas, J.P. %A Moulinier, C. %A Nepstad, K. %A Orduz, S. %A Sinègre, G. %T International indoor and outdoor evaluation of Bacillus sphaericus products : complexity of standardizing outdoor protocols %D 1997 %L fdi:010012152 %G ENG %J Journal of the American Mosquito Control Association %@ 8756-971X %K INSECTICIDE BIOLOGIQUE ; EFFICACITE ; SOUCHE ; ETUDE COMPARATIVE %K PROTOCOLE ; POPULATION LARVAIRE %N 3 %P 218-226 %U https://www.documentation.ird.fr/hor/fdi:010012152 %> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_47-48/010012152.pdf %V 13 %W Horizon (IRD) %X Only one #Bacillus sphaericus$ strain, strain 2362, is currently used commercially to control #Culex$ larval populations. A reliable methodology, easily used, was developed to identify new strains for field application. Larvicidal activities of 3 highly mosquitocidal strains, strains C3-41, Mal, and LB24, previously selected in the laboratory, were compared with that of strain 2362 in tropical and European countries. The following steps were performed : production and titration of acetonic powders from these 4 strains on local #Culex$ species, survey of initial and residual activity under standardized indoor and outdoor conditions, and evaluation of the efficacity of liquid formulations of the 4 strains in natural breeding sites of #Culex$. In indoor conditions, strain C3-41 showed the highest activity on both #Culex pipiens$ and #Culex quinquefasciatus$; strain Mal was the least active. The residual activity causing 80% mortality differed from 20 to 90 days according to the strains and the country. Outdoor experiments with powder (0,02-1,6 mg/liter) were performed and the initial toxicities were similar in all cases. Residual activities were different, from 6 to 95 days posttreatment. Liquid formulations were applied to larval habitats (from 0,1 to 10 g/m2). In tropical countries, larval recolonization in cesspits or ponds occured after 10-35 days. In Europe, higher doses were needed in polluted water than in clear water (from 3 to 10 liter/ha) for the same control, and the time before 80% residual activity was reached was less than 9-12 days. However, in cesspits, residual activity could be observed for 12 days to 5 mo. A strain 3-5 times more active than the others in bioassays is not significantly detectable from those strains in field trials. (Résumé d'auteur) %$ 052LUTIN01