Dwivedi P.P. auteur aut IRD Patel B.K.C. auteur aut IRD Rees G.N. auteur aut IRD Ollivier Bernard auteur aut IRD text journalArticle eng text/pdf reformatted digital access A method for DNA sequencing of ribosomal RNA (rRNA) genes, amplified by polymerase chain reaction (PCR), using internal primers, designed on the basis of conserved regions of rRNA genes for determining a near complete sequence (99%) of the gene using an automated DNA sequencer (Applied Biosystem Incorporation, USA) is described. The procedure is extremely rapid as cloning of the gene is not required for sequence determination. In addition time consuming steps such as ethanol precipitation and hazardous steps such as phenol/chloroform extractions are excluded from the protocol for the purification of extension products after Taq cycle sequencing using the ABI dye terminator chemistry. The method has been successfully used for sequencing of the 16S and 18S rRNA genes of microbes which includes six members of domain Bacteria, one of domain Archaea and one belonging to Eukarya domain. (Résumé d'auteur) specialized BIOLOGIE MOLECULAIRE GENE ARN METHODE D'ANALYSE PCR.REACTION DE POLYMERISATION EN CHAINE 020BIOL 36 9-12 1996 https://www.documentation.ird.fr/hor/fdi:010009566 [F B010009566] https://www.documentation.ird.fr/hor/fdi:010009566 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_45-46/010009566.pdf IRD - Base Horizon / Pleins textes 1997-04-07 2006-05-27 fdi:010009566 fre