Nyambi P.N., Lewi P., Peeters Martine, Janssens W., Heyndrickx L., Fransen K., Andries K., Vanden Haesevelde M., Heeney J., Piot P., Van Der Groen G. (1997). Study of the dynamics of neutralization escape mutants in a chimpanzee naturally infected with the Simian Immunodeficiency Virus SIVcpz-ant. Journal of Virology, 71 (3), p. 2320-2330. ISSN 0022-538X.
Titre du document
Study of the dynamics of neutralization escape mutants in a chimpanzee naturally infected with the Simian Immunodeficiency Virus SIVcpz-ant
Année de publication
1997
Auteurs
Nyambi P.N., Lewi P., Peeters Martine, Janssens W., Heyndrickx L., Fransen K., Andries K., Vanden Haesevelde M., Heeney J., Piot P., Van Der Groen G.
Source
Journal of Virology, 1997,
71 (3), p. 2320-2330 ISSN 0022-538X
Here we report on the use of spectral map analysis of time-paired sequential neutralization data of 11 serum samples of a chimpanzee naturally infected with a simian immunodeficiency virus (SIVcpz-ant) and 8 primary consecutive SIVcpz-ant isolates, taken at about 4-month intervals. The analysis reveals the existence of three SIVcpz-ant isolate and serum neutralization clusters. Each cluster group virus isolates and/or sera based on similarities of their neutralization spectra. On average, neutralization escape mutants emerged after 15 months and mounted a neutralization response approximately 8 months later. The entire gp160 regions of eight consecutive isolates were sequenced and analyzed by a new statistical method called polygram, which allowed the deduction of amino acid sequence motifs of gp160 which were specific for SIVcpz-ant isolates belonging to the same isolate neutralization clusters. Changes in specific amino acid quadruplets in V1, V2, C3, V4, V5, and CD4 domains of gp120 and gp40 were seen to correlate with the neutralization clusters with most of the specific changes occuring in the V4 region. This method of analysis may facilitate an understanding of the study of the dynamic interplay between human immunodeficiency virus (HIV) and host neutralization responses as well as providing possible insights into mechanisms of persistance of HIV-1 related lentiviruses in their natural hosts. (Résumé d'auteur)
Plan de classement
Maladies sexuellement transmissibles [052MALTRA03]
Descripteurs
ETUDE EXPERIMENTALE ; VIRUS ; MUTATION ; ANIMAL DE LABORATOIRE ; GENETIQUE ; ANALYSE SPECTRALE ; IMMUNOLOGIE ; PROTEINE ; VIRUS SIV ; VIRUS HIV-1 ; GLYCOPROTEINE
Localisation
Fonds IRD [F B010008999]
Identifiant IRD
fdi:010008999
