Giraud Eric auteur aut IRD Brauman Alain auteur aut IRD Kéléke S. auteur aut IRD Gosselin Laurent auteur aut IRD Raimbault Maurice auteur aut IRD text bookSection eng text/pdf reformatted digital access An amylolytic lactic acid bacterium, identified as Lactobacillus plantarum, was isolated from cassava roots (Manihot esculenta var. Ngansa) during retting. Cultured on starch, the strain displayed a growth rate of 0.43 per hour, a biomass yield of 0.19 g/g, and a lactate yield of 0.81 g/g. The growth kinetics were similar on starch and glucose. Enough enzyme was synthesized, and starch hydrolysis was not a limiting factor for growth. The synthesized amylolytic enzyme was purified by fractionated precipitation with ammonium sulfate and by anion exchange chromatography. It was identified as an alpha-amylase with an optimal pH of 5.5 and an optimal temperature of 65°C. The use of such a strain as a cassava fermentation starter for gari production had the following effects : a change from a heterofermentative pattern observed in natural fermentation to a homofermentation one, a lower final pH, a faster pH decline rate, and a greater production of lactic acid (50 g/kg of dry matter). (Résumé d'auteur) specialized FERMENTATION AMIDON GLUCOSE MICROBIOLOGIE BACTERIE MILIEU DE CULTURE ENZYME PURIFICATION PH TEMPERATURE ACTIVITE ENZYMATIQUE AMYLASE ACIDE LACTIQUE 084FERMEN Dufour D. ed. edt IRD O'Brien G.M. ed. edt IRD Best R. ed. edt IRD 271 210-220 Montpellier (FRA) CIRAD Cali CIAT 1996 271 https://www.documentation.ird.fr/hor/fdi:010008964 958-9439-88-8 [F B010008964] https://www.documentation.ird.fr/hor/fdi:010008964 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_45-46/010008964.pdf IRD - Base Horizon / Pleins textes 1997-03-12 2006-05-27 fdi:010008964 fre