Schmeda-Hirschmann G. auteur aut IRD Razmilic I. auteur aut IRD Sauvain Michel auteur aut IRD Moretti Christian auteur aut IRD Munoz V. auteur aut IRD Ruiz E. auteur aut IRD Balanza E. auteur aut IRD Fournet Alain auteur aut IRD text journalArticle eng text/pdf reformatted digital access The activity of jatrogrossidione, the main diterpene of Jatropha grossidentata and jatrophone from Jatropha isabellii was determined against Leishmania and Trypanosoma cruzi strains in vitro as well as against Leishmania amazonensis in vivo. Jatrogrossidione showed a strong in vitro leishmanicidal and trypanocidal activity with IC100 of 0.75 and 1.5-5.0 microg/mL, respectively. Under similar conditions, the IC100 of glucantime, ketoconazole and pentamidine towards Leishmania strains were >100, 50-100 and 1 microg/mL, respectively. The IC50 of jatrogrossidione was <0.25 microg/mL against amastigote forms of Leishmania infecting macrophages, with toxicity at concentrations higher than 0.5 microg/mL. BALB/c mice infected with L. amazonensis strain PH 8 were treated 24 h after infection with jatrogrossidione and jatrophone for 13 consecutive days. Jatrophone at 25 mg/kg/day subcutaneously administered was significantly active (p<0.05) against the virulent strain PH 8 of L. amazonensis ; it was more active than glucantime at 112 mg Sb per kg/day. Subcutaneous administration of jatrophone, however, proved to be too toxic under our assay conditions. Assays of single local treatment on the footpad infection 2 weeks after inoculation of L. amazonensis indicated that jatrogrossidione and jatrophone were inactive at the selected doses. (Résumé d'auteur) specialized LEISHMANIOSE MALADIE DE CHAGAS PARASITE TRAITEMENT MEDICAL LUTTE PLANTE MEDICINALE MEDICAMENT ETHNOMEDECINE COMPOSE ORGANIQUE EXTRACTION TERPENE JATROGROSSIDIONE JATROPHONE ACTIVITE ANTIPROTOZOAIRE 052PHLEIS03 076PLAMED03 10 375-378 1996 0951-418X https://www.documentation.ird.fr/hor/fdi:010007085 10.1002/(SICI)1099-1573(199608)10:5<375::AID-PTR847>3.0.CO;2-# 0951-418X [F B010007085] https://www.documentation.ird.fr/hor/fdi:010007085 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_45-46/010007085.pdf IRD - Base Horizon / Pleins textes 1996-10-02 2015-05-06 fdi:010007085 fre