Horizon 1 1 17 ACLN : Articles dans des revues avec comité de lecture non répertoriées par l'AERES Phytotherapy Research 375-378 LEISHMANIOSE MALADIE DE CHAGAS PARASITE TRAITEMENT MEDICAL LUTTE PLANTE MEDICINALE MEDICAMENT ETHNOMEDECINE COMPOSE ORGANIQUE EXTRACTION TERPENE JATROGROSSIDIONE JATROPHONE ACTIVITE ANTIPROTOZOAIRE 1996 fdi:010007085 ENG Phytotherapy Research 0951-418X 10.1002/(SICI)1099-1573(199608)10:5<375::AID-PTR847>3.0.CO;2-# https://www.documentation.ird.fr/hor/fdi:010007085 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_45-46/010007085.pdf 10 Horizon (IRD) The activity of jatrogrossidione, the main diterpene of #Jatropha grossidentata$ and jatrophone from #Jatropha isabellii$ was determined against #Leishmania$ and #Trypanosoma cruzi$ strains in vitro as well as against #Leishmania amazonensis$ in vivo. Jatrogrossidione showed a strong in vitro leishmanicidal and trypanocidal activity with IC100 of 0.75 and 1.5-5.0 microg/mL, respectively. Under similar conditions, the IC100 of glucantime, ketoconazole and pentamidine towards #Leishmania$ strains were >100, 50-100 and 1 microg/mL, respectively. The IC50 of jatrogrossidione was <0.25 microg/mL against amastigote forms of #Leishmania$ infecting macrophages, with toxicity at concentrations higher than 0.5 microg/mL. BALB/c mice infected with #L. amazonensis$ strain PH 8 were treated 24 h after infection with jatrogrossidione and jatrophone for 13 consecutive days. Jatrophone at 25 mg/kg/day subcutaneously administered was significantly active (p<0.05) against the virulent strain PH 8 of #L. amazonensis$ ; it was more active than glucantime at 112 mg Sb per kg/day. Subcutaneous administration of jatrophone, however, proved to be too toxic under our assay conditions. Assays of single local treatment on the footpad infection 2 weeks after inoculation of #L. amazonensis$ indicated that jatrogrossidione and jatrophone were inactive at the selected doses. (Résumé d'auteur) 052PHLEIS03 ; 076PLAMED03