Horizon 1 1 10 C-ACTI : Communications avec actes dans un congrès international Proceedings of the 2nd international conference on insect pests in the urban environment Insect Pests in the Urban Environment : International Conference 640 ENTOMOLOGIE MEDICALE INSECTICIDE CHIMIQUE RESISTANCE VARIABILITE GENETIQUE POLYMORPHISME ENZYMATIQUE COTE D'IVOIRE BURKINA FASO 1996 1996/07/07-10 Exeter BPC Weatons fdi:010007010 ENG https://www.documentation.ird.fr/hor/fdi:010007010 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_45-46/010007010.pdf Horizon (IRD) Larval susceptibility to organophosphates (OP), carbamates (CARB) and pyrethroids (PYR) was investigated in #Culex quinquefasciatus$ from Côte d'Ivoire and Burkina Faso. A total of 33 populations collected in 25 cities were tested. The resistance of these natural field populations was compared to a susceptible reference strain under the same conditions. In Côte d'Ivoire, populations showed a heterogeneous response to OP and CARB. A range of 40 %-98 % of larvae had a low resistance level to both chlorpyrifos (2-8x) and propoxur (1-4x). The remaining 2 % to 60 % of larvae displayed a high level of cross-resistance between chlorpyrifos (15-30x) and propoxur (>700x). Biochemical studies showed that low level resistance to OP was due to A2-B2 overproduced esterases and that cross-resistance to OP and CARB was conferred by an insensitive acetylcholinesterase (AChE). This AChE provided a lower resistance to temephos (10x). In Burkina Faso, populations were slightly resistant to OP (1-3x) and not to CARB. The esterases A2-B2 were only present at 50 % frequency. In contrast, PYR-resistance was similar between the two countries. All populations were resistant to either permethrin (20-80x) and deltamethrin (15-40x). Bioassays using piperonyl butoxide (PB) and biochemical studies showed that PYR-resistance involved increased metabolism by mixed function oxidases. Knowing that synergism of PB did not completely suppress resistance and that adults did not show a knockdown effect with high permethrin concentrations, it is likely that PYR-resistance was also due to a Kdr gene. (Résumé d'auteur) 052CULARB