Borsa Philippe auteur aut IRD Coustau C. auteur aut IRD text journalArticle eng text/pdf reformatted digital access The homologue of the resistance to dieldrin gene (Rdl) in Drosophila melanogaster was cloned and sequenced in the scolytid beetle Hypothenemus hampei, a coffee pest resistant to cyclodiene insecticides in New Caledonia. The amino acid sequence of the Rdl exon no. 7 protein product in H. hampei was identical to that in D. melanogaster and showed the same amino acid change as that characterizing susceptible vs. resistant D. melanogaster. Samples from natural H. hampei populations (from Asia, the Pacific Islands, Africa and Central America), from reference susceptible (S) and resistant (R) laboratory strains, and from their hybrid progenies, were analysed at the Rdl locus using single-stranded DNA conformation polymorphism on polymerase chain reaction products. The susceptible allele was the only allele present in all samples from natural populations except in the only resistant population known to date (Ponerihouen, New Caledonia). Females and some males obtained as F1 from R X S crosses were heterozygous at the Rdl locus, confirming that this local mate competing species is diplo-diploid. (Résumé d'auteur) specialized ENTOMOLOGIE APPLIQUEE INSECTE NUISIBLE CAFE INSECTICIDE CHIMIQUE SENSIBILITE RESISTANCE GENETIQUE ADN POLYMORPHISME 076RAVPLA 76 124-129 1996 0018-067X https://www.documentation.ird.fr/hor/fdi:010006167 10.1038/hdy.1996.19 0018-067X [F B010006167] https://www.documentation.ird.fr/hor/fdi:010006167 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_45-46/010006167.pdf IRD - Base Horizon / Pleins textes 1996-01-01 2009-05-29 fdi:010006167 fre