@article{PAR00026390,
  title = {{S}tatistical optimization and partial characterization of xylanases produced by {S}treptomyces sp. {S}1{M}3{I} using olive pomace as a fermentation substrate},
  author = {{M}edouni-{H}aroune, {L}. and {M}edouni-{A}drar, {S}. and {H}oufani, {A}. {A}. and {B}ouiche, {C}. and {A}zzouz, {Z}. and {R}oussos, {S}evastianos and {D}esseaux, {V}. and {M}adani, {K}. and {K}echa, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{X}ylanase production by {S}treptomyces sp. {S}1{M}3{I} was optimized by response surface methodology ({RSM}), followed by a partial characterization of these enzymes. {O}live pomace was used as a substrate for growing {S}treptomyces sp. {S}1{M}3{I} in submerged fermentation. {E}ffects of incubation time, p{H}, temperature, carbon source, nitrogen source, and inoculum size on xylanase production were studied, through the one-factor-at-a-time method. {T}hen, a 3(3)-factorial experimental design with {RSM} and the {B}ox-{B}ehnken design was investigated for the major influence factors. {M}aximum xylanase production (11.28 {U}/m{L}) was obtained when the strain was grown in mineral medium supplemented with 3% (w/v) of olive pomace powder and 0.3% (w/v) of ammonium sulfate, at a p{H} 7.4 and an incubation temperature of 40 & {DEG};{C}. {T}he xylanases in the supernatant degraded all tested substrates, with higher activity for the low-viscosity wheat arabinoxylan substrate. {T}wo xylanases with close molecular masses were detected by zymogram analysis: {X}yl-1 and {X}yl-2 with molecular masses of 24.14 k{D}a and 27 k{D}a, respectively. {T}he optimization of enzyme production parameters of {S}treptomyces sp. {S}1{M}3{I} and the characterization of these enzymes are prerequisites to enhancing xylanase production yield, which is crucial for further biotechnological processes.},
  keywords = {{A}ctinobacteria ; {X}ylanase ; {O}live pomace ; {O}ptimization ; {C}haracterization},
  booktitle = {},
  journal = {{A}pplied {B}iochemistry and {B}iotechnology},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[ p.]},
  ISSN = {0273-2289},
  year = {2023},
  DOI = {10.1007/s12010-023-04660-1},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00026390},
}