@article{PAR00024851,
  title = {{H}ighly specific and rapid detection of hepatitis c virus using {RT}-{LAMP}-coupled {CRISPR}-{C}as12 assay},
  author = {{K}ham-{K}jing, {N}. and {N}go-{G}iang-{H}uong, {N}icole and {T}ragoolpua, {K}. and {K}hamduang, {W}. and {H}ongjaisee, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}epatitis {C} virus ({HCV}) infection can be cured with pan-genotypic direct-acting antiviral agents. {H}owever, identifying individuals with current hepatitis {C} remains a major challenge, especially in resource-limited settings where access to or availability of molecular tests is still limited. {T}he goal of this study was to develop and validate a molecular assay for the rapid detection of {HCV} {RNA} in resource-limited settings. {I}t is based on a combination of reverse transcription loop-mediated isothermal amplification ({RT}-{LAMP}) with the clustered regularly interspaced short palindromic repeats-{CRISPR}-associated protein 12a ({CRISPR}-{C}as12a) cleavage assay that allows the recognition of specific {HCV} nucleic acid sequences. {A}mplified products after the cleavage reactions can be visualized on lateral flow strips or measured with a fluorescence detector. {W}hen tested on clinical samples from individuals infected with {HCV}, {HIV}, or {HBV}, or from healthy donors, the {RT}-{LAMP}-coupled {CRISPR}-{C}as12 assay yielded 96% sensitivity, 100% specificity, and 97% agreement as compared to the reference method ({R}oche {COBAS} {A}mpli{P}rep/{COBAS} {T}aq{M}an {HCV} {T}est). {T}his assay could detect {HCV} {RNA} concentrations as low as 10 ng/mu {L} (an estimated 2.38 {L}og(10) {IU}/m{L}). {T}herefore, this sensitive and specific assay may represent an affordable and reliable point-of-care test for the identification of individuals with active hepatitis {C} in low-resource settings.},
  keywords = {hepatitis {C} virus ; {HCV} {RNA} ; chronic hepatitis {C} ; point-of-care testing ; {RT}-{LAMP} ; {CRISPR}-{C}as12 ; lateral flow-based assay ; fluorescence-based ; assay},
  booktitle = {},
  journal = {{D}iagnostics},
  volume = {12},
  numero = {7},
  pages = {1524 [11 p.]},
  year = {2022},
  DOI = {10.3390/diagnostics12071524},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00024851},
}