Baron S. A., Mediannikov Oleg, Abdallah R., Yimagou E. K., Medkour Hacène, Dubourg G., Elamire Y., Afouda P., Ngom II, Angelakis E., Davoust B., Diatta Georges, Barciela A., Hernandez-Aguilar R. A., Sokhna Cheikh, Caputo A., Levasseur A., Rolain J. M., Raoult D. (2021). Multidrug-resistant Klebsiella pneumoniae clones from wild chimpanzees and termites in Senegal. Antimicrobial Agents and Chemotherapy, 65 (9), e02557-20 [p.]. ISSN 0066-4804.
Titre du document
Multidrug-resistant Klebsiella pneumoniae clones from wild chimpanzees and termites in Senegal
Année de publication
2021
Auteurs
Baron S. A., Mediannikov Oleg, Abdallah R., Yimagou E. K., Medkour Hacène, Dubourg G., Elamire Y., Afouda P., Ngom II, Angelakis E., Davoust B., Diatta Georges, Barciela A., Hernandez-Aguilar R. A., Sokhna Cheikh, Caputo A., Levasseur A., Rolain J. M., Raoult D.
Source
Antimicrobial Agents and Chemotherapy, 2021,
65 (9), e02557-20 [p.] ISSN 0066-4804
Antibiotic resistance genes exist naturally in various environments far from human usage. Here, we investigated multidrug-resistant Klebsiella pneumoniae, a common pathogen of chimpanzees and humans. We screened antibiotic-resistant K pneumoniae from 48 chimpanzee stools and 38 termite mounds (n=415 samples) collected in protected areas in Senegal. The microsatellite method was used to identify chimpanzee individuals (n =13). Whole-genome sequencing was performed on K pneumoniae complex isolates to identify antibiotic-resistant genes and characterize clones. We found a high prevalence of carbapenem-resistant K. pneumoniae among chimpanzee isolates (18/48 samples from 7/13 individuals) and ceftriaxone resistance among both chimpanzee individuals (19/48) and termite mounds (7/415 termites and 3/38 termite mounds). The bla(OXA-48) and the bla(KPC-2) genes were carried by international pOXA-48 and pKPC-2 plasmids, respectively. The ESBL plasmid carried bla(CTX-M)(-15), bla(TEM-18), and bla(OXA)(-1), genes. Genome sequencing of 56 isolates identified two major clones associated with hospital-acquired infections of K. pneumoniae (ST307 and ST147) in chimpanzees and termites, suggesting circulation of strains between the two species, as chimpanzees feed on termites. The source and selection pressure of these clones in this environment need to be explored.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
;
Biologie du sol [074]
;
Sciences du monde animal [080]
;
Biotechnologies [084]
Description Géographique
SENEGAL
Identifiant IRD
PAR00023384
