@article{PAR00021771,
  title = {{L}arge tandem duplications affect gene expression, 3{D} organization, and plant-pathogen response},
  author = {{P}icart-{P}icolo, {A}. and {G}rob, {S}. and {P}icault, {N}. and {F}ranek, {M}. and {L}lauro, {C}. and {H}alter, {T}. and {M}aier, {T}. {R}. and {J}obet, {E}. and {D}escombin, {J}. and {Z}hang, {P}. {P}. and {P}aramasivan, {V}. and {B}aum, {T}. {J}. and {N}avarro, {L}. and {D}vorackova, {M}. and {M}irouze, {M}arie and {P}ontvianne, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}apid plant genome evolution is crucial to adapt to environmental changes. {C}hromosomal rearrangements and gene copy number variation ({CNV}) are two important tools for genome evolution and sources for the creation of new genes. {H}owever, their emergence takes many generations. {I}n this study, we show that in {A}rabidopsis thaliana, a significant loss of ribosomal {RNA} (r{RNA}) genes with a past history of a mutation for the chromatin assembly factor 1 ({CAF}1) complex causes rapid changes in the genome structure. {U}sing long-read sequencing and microscopic approaches, we have identified up to 15 independent large tandem duplications in direct orientation ({TDDO}s) ranging from 60 kb to 1.44 {M}b. {O}ur data suggest that these {TDDO}s appeared within a few generations, leading to the duplication of hundreds of genes. {B}y subsequently focusing on a line only containing 20% of r{RNA} gene copies (20r{DNA} line), we investigated the impact of {TDDO}s on 3{D} genome organization, gene expression, and cytosine methylation. {W}e found that duplicated genes often accumulate more transcripts. {A}mong them, several are involved in plant-pathogen response, which could explain why the 20r{DNA} line is hyper-resistant to both bacterial and nematode infections. {F}inally, we show that the {TDDO}s create gene fusions and/or truncations and discuss their potential implications for the evolution of plant genomes.},
  keywords = {},
  booktitle = {},
  journal = {{G}enome {R}esearch},
  volume = {30},
  numero = {11},
  pages = {1583--1592},
  ISSN = {1088-9051},
  year = {2020},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00021771},
}