@article{PAR00021737,
  title = {{A}n earliest endosymbiont, {W}olbachia massiliensis sp. nov., strain {PL}13 from the bed bug ({C}imex hemipterus), type strain of a new supergroup {T}},
  author = {{L}aidoudi, {Y}. and {L}evasseur, {A}. and {M}edkour, {H}. and {M}aaloum, {M}. and {B}en {K}hedher, {M}. and {S}ambou, {M}. and {B}assene, {H}. and {D}avoust, {B}. and {F}enollar, {F}. and {R}aoult, {D}. and {M}ediannikov, {O}leg},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he symbiotic {W}olbachia are the most sophisticated mutualistic bacterium among all insect-associated microbiota. {W}olbachia-insect relationship fluctuates from the simple facultative/parasitic to an obligate nutritional-mutualistic association as it was the case of the bedbug-{W}olbachia from {C}imex lectularius. {U}nderstanding this association may help in the control of associated arthropods. {G}enomic data have proven to be reliable tools in resolving some aspects of these symbiotic associations. {A}lthough, {W}olbachia appear to be fastidious or uncultivated bacteria which strongly limited their study. {H}ere we proposed {D}rosophila {S}2 cell line for the isolation and culture model to study {W}olbachia strains. {W}e therefore isolated and characterized a novel {W}olbachia strain associated with the bedbug {C}imex hemipterus, designated as w{C}hem strain {PL}13, and proposed {W}olbachia massiliensis sp. nov. strain w{C}hem-{PL}13 a type strain of this new species from new supergroup {T}. {P}hylogenetically, {T}-supergroup was close to {F} and {S}-supergroups from insects and {D}-supergroup from filarial nematodes. {W}e determined the 1,291,339-bp genome of w{C}hem-{PL}13, which was the smallest insect-associated {W}olbachia genomes. {O}verall, the w{C}hem genome shared 50% of protein coding genes with the other insect-associated facultative {W}olbachia strains. {T}hese findings highlight the diversity of {W}olbachia genotypes as well as the {W}olbachia-host relationship among {C}imicinae subfamily. {T}he w{C}hem provides folate and riboflavin vitamins on which the host depends, while the bacteria had a limited translation mechanism suggesting its strong dependence to its hosts. {H}owever, the clear-cut distinction between mutualism and parasitism of the w{C}hem in {C}. hemipterus cannot be yet ruled out.},
  keywords = {{W}olbachia ; bedbug ; {C}imex hemipterus ; isolation ; culture ; genomics ; {B}-vitamins},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {M}olecular {S}ciences},
  volume = {21},
  numero = {21},
  pages = {8064 [18p.]},
  year = {2020},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00021737},
}