%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Andre, S.
%A Raja, Z.
%A Humblot, V.
%A Piesse, C.
%A Foulon, T.
%A Sereno, Denis
%A Oury, Bruno
%A Ladram, A.
%T Functional characterization of temporin-she, a new broad-spectrum antibacterial and leishmanicidal temporin-SH paralog from the Sahara frog (Pelophylax saharicus)
%D 2020
%L PAR00021702
%G ENG
%J International Journal of Molecular Sciences
%K frog antimicrobial peptide ; temporin-SHe ; broad-spectrum activity ; bacteria ; parasites ; secondary structure ; membrane disrupting mechanism ; scanning electron microscopy
%M ISI:000580769700001
%N 18
%P 6713 [19 ]
%R 10.3390/ijms21186713
%U https://www.documentation.ird.fr/hor/PAR00021702
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-11/010079861.pdf
%V 21
%W Horizon (IRD)
%X Amphibian skin is a promising natural resource for antimicrobial peptides (AMPs), key effectors of innate immunity with attractive therapeutic potential to fight antibiotic-resistant pathogens. Our previous studies showed that the skin of the Sahara Frog (Pelophylax saharicus) contains broad-spectrum AMPs of the temporin family, named temporins-SH. Here, we focused our study on temporin-SHe, a temporin-SHd paralog that we have previously identified in this frog but was never structurally and functionally characterized. We synthesized and determined the structure of temporin-SHe. This non-amphipathic alpha-helical peptide was demonstrated to strongly destabilize the lipid chain packing of anionic multilamellar vesicles mimicking bacterial membranes. Investigation of the antimicrobial activity revealed that temporin-SHe targets Gram-negative and Gram-positive bacteria, including clinical isolates of multi-resistant Staphylococcus aureus strains. Temporin-SHe exhibited also antiparasitic activity toward differentLeishmaniaspecies responsible for visceral leishmaniasis, as well as cutaneous and mucocutaneous forms. Functional assays revealed that temporin-SHe exerts bactericidal effects with membrane depolarization and permeabilization, via a membranolytic mechanism observed by scanning electron microscopy. Temporin-SHe represents a new member of the very limited group of antiparasitic temporins/AMPs. Despite its cytotoxicity, it is nevertheless an interesting tool to study the AMP antiparasitic mechanism and design new antibacterial/antiparasitic agents.
%$ 020 ; 084 ; 052 ; 080