@article{PAR00019828,
  title = {{C}yprinid herpesvirus 3 evolves in vitro through an assemblage of haplotypes that alternatively become dominant or under-represented},
  author = {{K}lafack, {S}. and {F}iston-{L}avier, {A}. {S}. and {B}ergmann, {S}. {M}. and {H}ammoumi, {S}. and {S}chroder, {L}. and {F}uchs, {W}. and {L}usiastuti, {A}. and {L}ee, {P}. {Y}. and {H}eredia, {S}. {V}. and {G}osselin-{G}renet, {A}. {S}. and {A}varre, {J}ean-{C}hristophe and {A}moyal, {C}. and {A}mraoui, {H}. and {B}igourdan, {A}. and {B}oujandir, {I}. and {B}ueno, {O}. and {C}otteux, {B}. and de {S}an {N}icolas, {N}. and {D}elattre, {R}. and {D}iouf, {M}. {B}. and {D}ussaut, {R}. and {G}awron, {K}. and {I}mbert, {J}. {B}. and {M}ouilhi, {H}. and {N}ifaut, {E}. and {P}ages, {A}. and {P}laton, {L}. and {S}anchez, {S}. and {S}imon, {L}. and {S}obry, {H}. and {S}ouche, {M}. and {T}aillefer, {B}. and {G}uemgne, {E}. {N}. and {S}acchi, {O}. and {D}iouf, {A}. and {O}uzerdine, {A}. {L}. and {D}urand, {F}. and {B}rooke, {J}. and {C}remaschi, {J}. and {M}assaviol, {M}. and {H}amouma, {M}. and {S}alma, {M}. and {S}oulie, {M}. and {D}elorme, {Q}. and {V}irgoulay, {T}. and {K}lein, {V}alentin and {A}ntoine, {Y}. and {B}erne, {A}. and {G}aal, {C}. and {M}aster {S}tudent {C}onsortium,},
  editor = {},
  language = {{ENG}},
  abstract = {{V}iruses are able to evolve in vitro by mutations after serial passages in cell cultures, which can lead to either a loss, or an increase, of virulence. {C}yprinid herpesvirus 3 ({C}y{HV}-3), a 295-kb double-stranded {DNA} virus, is the etiological agent of the koi herpesvirus disease ({KHVD}). {T}o assess the influence of serial passages, an isolate of {C}y{HV}-3 ({KHV}-{T}) was passaged 99 times onto common carp brain ({CCB}) cells, and virus virulence was evaluated during passages through the experimental infections of common carp. {A}fter 78 {CCB} passages, the isolate was much less virulent than the original form. {A} comparative genomic analysis of these three forms of {KHV}-{T} ({P}0, {P}78 and {P}99) revealed a limited number of variations. {T}he largest one was a deletion of 1363 bp in the predicted {ORF}150, which was detected in {P}78, but not in {P}99. {T}his unexpected finding was confirmed by conventional {PCR} and digital {PCR}. {T}he results presented here primarily suggest that, {C}y{HV}-3 evolves, at least in vitro, through an assemblage of haplotypes that alternatively become dominant or under-represented.},
  keywords = {{C}yprinid herpesvirus 3 ; cell culture passages ; virus evolution ; haplotype sequence},
  booktitle = {},
  journal = {{V}iruses},
  volume = {11},
  numero = {8},
  pages = {art. 754 [12 p.]},
  year = {2019},
  DOI = {10.3390/v11080754},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00019828},
}