@article{PAR00013062,
  title = {{M}olecular signatures discriminating the male and the female sexual pathways in the pearl oyster {P}inctada margaritifera},
  author = {{T}eaniniuraitemoana, {V}. and {H}uvet, {A}. and {L}evy, {P}. and {G}aertner-{M}azouni, {N}abila and {G}ueguen, {Y}. and {L}e {M}oullac, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he genomics of economically important marine bivalves is studied to provide better understanding of the molecular mechanisms underlying their different reproductive strategies. {T}he recently available gonad transcriptome of the black-lip pearl oyster {P}inctada margaritifera is a novel and powerful resource to study these mechanisms in marine mollusks displaying hermaphroditic features. {I}n this study, {RNA}seq quantification data of the {P}. margaritifera gonad transcriptome were analyzed to identify candidate genes in histologically-characterized gonad samples to provide molecular signatures of the female and male sexual pathway in this pearl oyster. {B}ased on the {RNA}seq data set, stringent expression analysis identified 1,937 contigs that were differentially expressed between the gonad histological categories. {F}rom the hierarchical clustering analysis, a new reproduction model is proposed, based on a dual histo-molecular analytical approach. {N}ine candidate genes were identified as markers of the sexual pathway: 7 for the female pathway and 2 for the male one. {T}heir m{RNA} levels were assayed by real-time {PCR} on a new set of gonadic samples. {A} clustering method revealed four principal expression patterns based on the relative gene expression ratio. {A} multivariate regression tree realized on these new samples and validated on the previously analyzed {RNA}seq samples showed that the sexual pathway of {P}. margaritifera can be predicted by a 3-gene-pair expression ratio model of 4 different genes: pmarg-43476, pmarg-foxl2, pmarg-54338 and pmarg-fem1-like. {T}his 3-gene-pair expression ratio model strongly suggests only the implication of pmarg-foxl2 and pmarg-fem1-like in the sex inversion of {P}. margaritifera. {T}his work provides the first histo-molecular model of {P}. margaritifera reproduction and a gene expression signature of its sexual pathway discriminating the male and female pathways. {T}hese represent useful tools for understanding and studying sex inversion, sex differentiation and sex determinism in this species and other related species for aquaculture purposes such as genetic selection programs.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {10},
  numero = {3},
  pages = {e0122819 [20 p.]},
  ISSN = {1932-6203},
  year = {2015},
  DOI = {10.1371/journal.pone.0122819},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013062},
}