@article{PAR00012825,
  title = {{F}lavivirus reverse genetic systems, construction techniques and applications : a historical perspective},
  author = {{A}ubry, {F}. and {N}ougairede, {A}. and {G}ould, {E}. {A}. and de {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he study of flaviviruses, which cause some of the most important emerging tropical and sub-tropical human arbovirus diseases, has greatly benefited from the use of reverse genetic systems since its first development for yellow fever virus in 1989. {R}everse genetics technology has completely revolutionized the study of these viruses, making it possible to manipulate their genomes and evaluate the direct effects of these changes on their biology and pathogenesis. {T}he most commonly used reverse genetics system is the infectious clone technology. {W}hilst flavivirus infectious clones provide a powerful tool, their construction as full-length c{DNA} molecules in bacterial vectors can be problematic, laborious and time consuming, because they are often unstable, contain unwanted induced substitutions and may be toxic for bacteria due to viral protein expression. {T}he incredible technological advances that have been made during the past 30 years, such as the use of {PCR} or new sequencing methods, have allowed the development of new approaches to improve preexisting systems or elaborate new strategies that overcome these problems. {T}his review summarizes the evolution and major technical breakthroughs in the development of flavivirus reverse genetics technologies and their application to the further understanding and control of these viruses and their diseases.},
  keywords = {{F}lavivirus ; {R}everse genetics ; {I}nfectious clone},
  booktitle = {},
  journal = {{A}ntiviral {R}esearch},
  volume = {114},
  numero = {},
  pages = {67--85},
  ISSN = {0166-3542},
  year = {2015},
  DOI = {10.1016/j.antiviral.2014.12.007},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012825},
}