@article{PAR00012484,
  title = {{D}evelopment of a latex agglutination test with recombinant variant surface glycoprotein for serodiagnosis of surra},
  author = {{R}og{\'e}, {S}. and {B}aelmans, {R}. and {C}laes, {F}. and {L}ejon, {V}eerle and {G}uisez, {Y}. and {J}acquet, {D}. and {B}uscher, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}erodiagnosis of surra is commonly performed with the {CATT}/{T}rypanosoma evansi direct agglutination test. {T}his antibody detection test is based on lyophilised bloodstream form trypanosomes propagated in rats and presenting the predominant variant surface glycoprotein ({VSG}) {R}o{T}at 1.2 on their surface. {R}ecently, the {N}-terminal fragment of {VSG} {R}o{T}at 1.2 has been expressed as a recombinant protein in the yeast {P}ichia pastoris and showed diagnostic potential in {ELISA}. {T}his recombinant antigen has now been incorporated in a latex agglutination test, the r{LATEX}/{T}. evansi. {I}n this study, we compared the diagnostic accuracy of r{LATEX}/{T}. evansi and {CATT}/{T}. evansi with immune trypanolysis ({TL}) as reference test on a total of 1717 sera from camels, horses, bovines, water buffaloes, dogs and sheep. {T}he r{LATEX}/{T}. evansi displayed a slightly better agreement with {TL} than {CATT}/{T}. evansi (kappa [{K}] respectively 0.84 and 0.72). {T}he sensitivities of r{LATEX}/{T}. evansi (84.2%, 95% {CI} 80.8-87.1) and {CATT}/{T}. evansi (84.0%, 95% {CI} 80.6-87.0) were similar, but r{LATEX}/{T}. evansi was significantly more specific (97.7%, 95% {CI} 96.7-98.4) than {CATT}/{T}. evansi (89.4%; 95% {CI} 87.6-91.1). {W}e consider the r{LATEX}/{T}. evansi an alternative for the {CATT}/{T}. evansi, with the advantage that the use of a purified recombinant antigen leads to a more standardised diagnostic test with an improved specificity. {M}oreover, it eliminates the use of laboratory animals and can be easily scaled-up, e.g. in biofermentors.},
  keywords = {{T}rypanosoma evansi ; {S}urra ; {D}omestic animals ; {A}ntibody detection ; {R}o{T}at ; 1.2 ; {S}erodiagnosis ; {AFRIQUE} {DU} {NORD} ; {MOYEN} {ORIENT} ; {ASIE} ; {AMERIQUE} {LATINE}},
  booktitle = {},
  journal = {{V}eterinary {P}arasitology},
  volume = {205},
  numero = {3-4},
  pages = {460--465},
  ISSN = {0304-4017},
  year = {2014},
  DOI = {10.1016/j.vetpar.2014.08.026},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012484},
}