@article{PAR00009920,
  title = {{M}olecular and physiological study of the artificial maturation process in {E}uropean eel males: {F}rom brain to testis},
  author = {{P}enaranda, {D}. {S}. and {P}erez, {L}. and {G}allego, {V}. and {J}over, {M}. and {T}veiten, {H}. and {B}aloche, {S}. and {D}ufour, {S}ylvie and {A}sturiano, {J}. {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}uropean eel males can be artificially matured (1.5 {IU} h{CG}/g fish), but the regulatory mechanisms of their reproductive development are practically unknown. {S}permatogenic stages ({S}1-{S}6), biometric characters [eye index ({EI}), gonadosomatic index ({GSI}), hepatosomatic index ({HSI})] and sperm quality parameters (motility, viability and head spermatozoa morphometry) were analysed. {M}oreover, the present study evaluated the expression of {G}n{RH}s (mammal and chicken 11 {G}onadotropin {R}elease {H}ormone 1) and gonadotrophins ({FSH}p and {LHP}) during hormonal treatment, as well as 11-ketotestosterone (11-{KT}) and 17,20p-dihydroxy-4-pregnen-3-one (17,20 beta-{P}) plasma levels. {O}ne week was enough to observe the {S}2 of gonad development, but it was necessary to reach the 7th week of treatment to obtain animals that presented the most advanced stage of development ({S}6). {D}ifferential regulation of the two {G}n{RH} expressions was found, supporting the main role of m{G}n{RH} in the control of gonadotrophin release. {O}ne h{CG} injection was enough to dramatically decrease the {FSH} beta expression, being close to zero during the rest of the treatment. {LHP} expression and 17,20 beta-{P} registered a significant increase in the same stage of development, {S}3/4, confirming the role of this gonadotrophin in the last steps of maturation and 17,20 beta-{P} in the spermatozoa maturation. {T}he 11-{KT} increased with {GSI}, and the highest 11-{KT} values coincided with the advanced steps of spermatogenesis prior to spermiation. {B}eing consistent with the known role of the steroid in these processes. {F}urthermore, this study supports a role for 11-{KT} in stimulating eye growth, presenting high values when {E}l increased. {S}perm production was obtained from the 4th week of treatment, but it was in the 8th week when a significant increase was observed in sperm quality [viability, high motility (>75%)].},
  keywords = {{E}uropean eel ; {S}perm ; {G}n{RH} ; {G}onadotrophin ; 11-{KT} ; {T}estis},
  booktitle = {},
  journal = {{G}eneral and {C}omparative {E}ndocrinology},
  volume = {166},
  numero = {1},
  pages = {160--171},
  ISSN = {0016-6480},
  year = {2010},
  DOI = {10.1016/j.ygcen.2009.08.006},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00009920},
}